负载于SBA - 15介孔二氧化硅颗粒中的黄腐酚和异黄腐酚对B16F10黑色素瘤细胞的抗癌潜力

Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.

作者信息

Krajnović Tamara, Pantelić Nebojša Đ, Wolf Katharina, Eichhorn Thomas, Maksimović-Ivanić Danijela, Mijatović Sanja, Wessjohann Ludger A, Kaluđerović Goran N

机构信息

Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

Department of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Straße 2, 06217 Merseburg, Germany.

出版信息

Materials (Basel). 2022 Jul 19;15(14):5028. doi: 10.3390/ma15145028.

DOI:10.3390/ma15145028

PMID:35888494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9320346/

Abstract

Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from , have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated.

摘要

黄腐酚（XN）和异黄腐酚（IXN）是来自[具体来源未给出]的异戊烯基黄酮，已被证明具有抗肿瘤/抗癌保护、抗氧化、抗炎和抗血管生成特性。在本研究中，用不同量的黄腐酚和异黄腐酚负载介孔二氧化硅（SBA - 15），并通过标准分析方法进行表征。已评估负载到SBA - 15中的XN和IXN对恶性小鼠黑色素瘤B16F10细胞的抗癌潜力。当用含有黄腐酚的SBA - 15处理这些细胞时，观察到活性增加与XN的更高固定率相关。考虑到负载到SBA - 15中的XN量（由热重分析计算），观察到XN的抗肿瘤潜力有所提高（SBA - 15|XN2和SBA - 15|XN3的IC50分别为10.8±0.4和11.8±0.5μM；相比之下，游离XN的IC50为18.5±1.5μM）。固定化XN对抗肿瘤细胞的主要机制包括抑制增殖和自噬性细胞死亡。在所有研究的情况下，负载异黄腐酚的SBA - 15的MC值均超过300μg/mL。