克罗地亚神经侵袭性疾病患者中塔希纳正布尼亚病毒中和抗体的检测

Detection of Tahyna Orthobunyavirus-Neutralizing Antibodies in Patients with Neuroinvasive Disease in Croatia.

作者信息

Vilibic-Cavlek Tatjana, Stevanovic Vladimir, Savic Vladimir, Markelic Domagoj, Sabadi Dario, Bogdanic Maja, Kovac Snjezana, Santini Marija, Tabain Irena, Potocnik-Hunjadi Tanja, Ferencak Ivana, Skoda Ana Marija, Sankovic Ana, Barbic Ljubo

机构信息

Department of Virology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.

School of Medicine, University of Zagreb, 10000 Zagreb, Croatia.

出版信息

Microorganisms. 2022 Jul 18;10(7):1443. doi: 10.3390/microorganisms10071443.

DOI:10.3390/microorganisms10071443

PMID:35889162

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9316594/

Abstract

BACKGROUND

Tahyna orthobunyavirus (TAHV) is widely distributed in continental Europe. Very few studies have analyzed TAHV seroprevalence in Croatia. We analyzed the prevalence of TAHV RNA and antibodies in Croatian patients with neuroinvasive disease (NID).

METHODS

A total of 218 patients with unsolved NID detected during five consecutive arbovirus transmission seasons (April 2017-October 2021) were tested. Cerebrospinal fluid (CSF) and urine samples were tested for TAHV RNA using RT-PCR. In addition, CSF and serum samples were tested for TAHV antibodies using a virus neutralization test (VNT).

RESULTS

Clinical presentations in patients with NID were meningitis (141/64.7%), meningoencephalitis (56/25.7%), myelitis (8/3.7%), and 'febrile headache' (13/5.9%). TAHV RNA was not detected in any of the tested CSF or urine samples; however, TAHV-neutralizing (NT) antibodies were detected in 22/10.1% of patients. Detection of NT antibodies in the CSF of two patients presenting with meningitis suggested recent TAHV infection. TAHV seropositivity increased significantly with age, from 1.8% to 24.4%. There was no difference in seroprevalence between genders or areas of residence (urban, suburban/rural). The majority of seropositive patients (90.9%) resided in floodplains along the rivers in continental Croatia.

CONCLUSIONS

The presented results confirm that TAHV is present in Croatia. The prevalence and clinical significance of TAHV infection in the Croatian population have yet to be determined.

摘要

背景

塔希纳正布尼亚病毒（TAHV）在欧洲大陆广泛分布。在克罗地亚，很少有研究分析TAHV的血清流行率。我们分析了克罗地亚神经侵袭性疾病（NID）患者中TAHV RNA和抗体的流行情况。

方法

对在连续五个虫媒病毒传播季节（2017年4月至2021年10月）期间检测到的218例未确诊的NID患者进行了检测。使用逆转录聚合酶链反应（RT-PCR）检测脑脊液（CSF）和尿液样本中的TAHV RNA。此外，使用病毒中和试验（VNT）检测CSF和血清样本中的TAHV抗体。

结果

NID患者的临床表现为脑膜炎（141/64.7%）、脑膜脑炎（56/25.7%）、脊髓炎（8/3.7%）和“发热性头痛”（13/5.9%）。在任何检测的CSF或尿液样本中均未检测到TAHV RNA；然而，在22/10.1%的患者中检测到了TAHV中和（NT）抗体。两名表现为脑膜炎的患者的CSF中检测到NT抗体，提示近期感染TAHV。TAHV血清阳性率随年龄显著增加，从1.8%增至24.4%。性别或居住地区（城市、郊区/农村）之间的血清流行率没有差异。大多数血清阳性患者（90.9%）居住在克罗地亚大陆河流沿岸的洪泛区。