Rafarophe Co, Venture Research Center Suite 605, 194-41, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju 28160, Korea.
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Korea.
Molecules. 2021 Mar 13;26(6):1600. doi: 10.3390/molecules26061600.
Persimmon (), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals. However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied. In this study, extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system. DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L. Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon. Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues. These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.
柿子（）是一种在全球范围内常见且广泛分布的水果，由于存在具有药理活性的称为植物化学物质的化合物，因此具有多种生理作用。然而，其高分子量化合物，特别是多糖，尚未得到广泛研究。在这项研究中，通过乙醇分级，将提取物（DK）分为低分子量和高分子量部分（DK-L 和 DK-H），并通过体外系统研究它们对抗氧化、抗炎和抗皱活性的影响。与 DK 和 DK-L 相比，DK-H 中的中性糖、糖醛酸和多酚含量明显更高。此外，与 DK 和 DK-L 相比，DK-H 表现出明显改善的药理活性，如抗氧化、抗炎和抗皱特性，表明 DK-H 可能在介导柿子的有益作用方面发挥重要作用。糖组成分析和分子特征表明，DK-H 由一种富含半乳糖醛酸（GalA）的多糖组成，分子量>345 kDa，主要由 GalA 和少量中性糖和多酚残基组成。这些结果表明，生物活性部分 DK-H 可能是一种富含半乳糖醛酸的果胶多糖，含有少量的多酚残基，可能是制药和化妆品行业的新型候选物。
