Tian Jie, Ke Xiang-Hong, Yuan Yuan, Yang Wen-Xiang, Tang Xiao-Qiao, Pei Lan-Jie, Fan Jun, Zhuo Qin, Yang Xiao-Guang, Liu Jia-Fa, Fan Bo-Lin
Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China.
Key Laboratory of Trace Element Nutrition of National Health Commission, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Plants (Basel). 2022 Jul 12;11(14):1823. doi: 10.3390/plants11141823.
The aim of the current study was to evaluate the subchronic toxicity of GmDREB3 gene modified wheat in the third generation rats. SPF Wistar rats were fed with transgenic wheat diet (Gm), parental wheat diet (Jimai22) and AIN-93 rodent diet (Control), respectively, for two generations, to produce the third generation rats which were used for this study. The selected fresh weaned offspring rats (20/sex/group) were given the same diet as their parents for 13 weeks. No toxicity-related changes were observed in rats fed with Gm diet in the following respects: clinical signs, body weights, body weight gains, food consumption, food utilization rate, urinalysis, hematology, serum biochemistry and histopathology. The results from the present study demonstrated that 13 weeks consumption of Gm wheat did not cause any adverse effects in the third generation rats when compared with the corresponding Jimai22 wheat.
本研究的目的是评估GmDREB3基因修饰小麦对第三代大鼠的亚慢性毒性。将SPF级Wistar大鼠分别用转基因小麦日粮（Gm）、亲本小麦日粮（济麦22）和AIN-93啮齿动物日粮（对照）喂养两代，以产生用于本研究的第三代大鼠。选择新鲜断奶的子代大鼠（每组20只/性别），给予与它们父母相同的日粮，持续13周。在以下方面，用Gm日粮喂养的大鼠未观察到与毒性相关的变化：临床体征、体重、体重增加、食物消耗、食物利用率、尿液分析、血液学、血清生化和组织病理学。本研究结果表明，与相应的济麦22小麦相比，食用13周的Gm小麦对第三代大鼠未造成任何不良影响。
