Dow AgroSciences LLC, Indianapolis, IN, 46268, USA.
Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, MI, 48674, USA.
Regul Toxicol Pharmacol. 2018 Apr;94:57-69. doi: 10.1016/j.yrtph.2018.01.005. Epub 2018 Jan 6.
Event DAS-444Ø6-6 soybean is genetically modified (GM) to provide tolerance to 2,4-diclorophenoxyacetic acid (2,4-D), glyphosate, and glufosinate herbicides through expression of the AAD-12, 2mEPSPS, and PAT proteins, respectively. DAS-444Ø6-6 soybeans were evaluated for safety in subchronic rat feeding studies. The results from two previous subchronic rat feeding studies evaluating diets formulated with 20% inclusion of DAS-444Ø6-6 soybean meal (the latter also containing DAS-444Ø6-6 derived hulls and oil) did not show any treatment-related adverse effects. In 2017, to comply with recent guidance from EFSA, a third 90-day rat feeding study was conducted with Sprague-Dawley rats (16/sex/group) with diets formulated either with 15% or 30% w/w of toasted DAS-444Ø6-6 soybean meal. DAS-444Ø6-6 soybean hulls and oil were also added to the transgenic test diets at 1% or 2% w/w and 1.35% or 2.7%, respectively, for the low- and high-dose groups. No toxicologically significant effects were observed under the conditions of this study.
事件 DAS-444Ø6-6 大豆经过基因改良（GM），通过表达 AAD-12、2mEPSPS 和 PAT 蛋白，分别对 2,4-二氯苯氧乙酸（2,4-D）、草甘膦和草铵膦除草剂具有耐受性。DAS-444Ø6-6 大豆在亚慢性大鼠喂养研究中进行了安全性评估。之前两项评估含有 20% DAS-444Ø6-6 大豆粉的饲料配方的亚慢性大鼠喂养研究的结果[后者还含有 DAS-444Ø6-6 衍生的豆壳和油]没有显示出任何与处理相关的不良影响。2017 年，为了遵守 EFSA 的最新指南，用 Sprague-Dawley 大鼠（每组 16 只/性别）进行了第三次 90 天大鼠喂养研究，用 15%或 30% w/w 烤制的 DAS-444Ø6-6 大豆粉配制饲料。DAS-444Ø6-6 豆壳和油也分别以 1%或 2% w/w 和 1.35%或 2.7%的剂量添加到转基因测试饲料中，用于低剂量和高剂量组。在本研究条件下，未观察到具有毒理学意义的影响。
