Zhang Jie, Wang Jing, Fan Guixiu, Zhang Bingjie, Ma Guanglong, Xiao Haiyan, Wang Longgang
Key Laboratory of Applied Chemistry, Nano-Biotechnology Key Lab of Hebei Province, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.
Polymers (Basel). 2022 Jul 7;14(14):2787. doi: 10.3390/polym14142787.
Silver nanoparticles as photothermal agents have the problems of low stability and low photothermal conversion efficiency. Amphiphilic daptomycin can improve the stability of silver nanoparticles, thereby improving their photothermal conversion efficiency. Herein, daptomycin-biomineralized silver nanoparticles (Dap-AgNPs) were prepared by reducing silver nitrate with sodium borohydride in the presence of daptomycin as a stabilizer and biomineralizer. The Dap-AgNPs had good solution stability and peroxidase-like activity. Furthermore, the photothermal conversion efficiency of the Dap-AgNPs was as high as 36.8%. The Dap-AgNPs displayed good photothermal stability under irradiation. More importantly, the Dap-AgNPs showed good cell compatibility with HeLa cells and HT-29 cells without irradiation by 808-nanometer near-infrared light at a concentration of 0.5 mM, and the cell viability was greater than 85.0%. However, the Dap-AgNPs displayed significant anti-tumor ability with irradiation by 808-nanometer near-infrared light, which was due to the increasing temperature of the culture medium caused by the Dap-AgNPs. In conclusion, Dap-AgNPs have potential applications as photothermal agents in the treatment of tumors.
作为光热剂的银纳米颗粒存在稳定性低和光热转换效率低的问题。两亲性达托霉素可以提高银纳米颗粒的稳定性，从而提高其光热转换效率。在此，在作为稳定剂和生物矿化剂的达托霉素存在下，通过硼氢化钠还原硝酸银制备了达托霉素生物矿化银纳米颗粒（Dap-AgNPs）。Dap-AgNPs具有良好的溶液稳定性和类过氧化物酶活性。此外，Dap-AgNPs的光热转换效率高达36.8%。Dap-AgNPs在光照下表现出良好的光热稳定性。更重要的是，在浓度为0.5 mM时，Dap-AgNPs在未受808纳米近红外光照射的情况下，与HeLa细胞和HT-29细胞表现出良好的细胞相容性，细胞活力大于85.0%。然而，Dap-AgNPs在808纳米近红外光照射下表现出显著的抗肿瘤能力，这是由于Dap-AgNPs导致培养基温度升高所致。总之，Dap-AgNPs作为光热剂在肿瘤治疗中具有潜在应用。
