Bernabe-Dones Raul D, Gonzalez-Pons Maria, Villar-Prados Alejandro, Lacourt-Ventura Mercedes, Rodríguez-Arroyo Heriberto, Fonseca-Williams Sharon, Velazquez Francisco E, Diaz-Algorri Yaritza, Lopez-Diaz Sofia M, Rodríguez Nayra, Yamamura Yasuhiro, Cruz-Correa Marcia
School of Health Professions, University of Puerto Rico Medical Sciences Campus, San Juan, PR 00935, USA; Division of Gastrointestinal Oncology, University of Puerto Rico Comprehensive Cancer Center, San Juan, PR 00935, USA.
Division of Gastrointestinal Oncology, University of Puerto Rico Comprehensive Cancer Center, San Juan, PR 00935, USA.
Gastroenterol Res Pract. 2016;2016:7896716. doi: 10.1155/2016/7896716. Epub 2016 Jan 19.
The role of Human Papillomavirus (HPV) in colorectal carcinogenesis remains elusive. Based on the high incidence of HPV-associated malignancies among Puerto Rican Hispanics, this study aimed to assess the prevalence of HPV infection and viral integration in colorectal tissues in order to evaluate its putative role in colorectal cancer (CRC). In this case-control study, the prevalence of HPV infection in CRC (cases n = 45) and normal colon mucosa from cancer-free subjects (controls n = 36) was assessed by a nested PCR strategy. HPV-16 genotyping was performed in HPV-positive tissues and the physical status of the HPV-16 genome was determined by E2 detection. HPV was detected in 19 of 45 (42.2%) CRC cases (mean age 61.1 ± 10.7 years, 24 males) and in 1 of 36 (2.8%) controls (mean age 60.9 ± 9.6 years, 24 males) with an OR = 25.58 (95% CI 3.21 to 203.49). HPV-16 was detected in 63.2% of the HPV-positive colorectal tumors; genome integration was observed in all HPV-16 positive cases. This is the first report showing the high prevalence of HPV infections in Caribbean Hispanic colorectal tumors. Despite evidence of HPV integration into the host genome, further mechanistic analysis examining HPV oncoprotein expression and the putative role of these oncoproteins in colorectal carcinogenesis is warranted.
人乳头瘤病毒（HPV）在结直肠癌发生过程中的作用仍不清楚。基于波多黎各裔西班牙人中HPV相关恶性肿瘤的高发病率，本研究旨在评估结直肠组织中HPV感染和病毒整合的患病率，以评估其在结直肠癌（CRC）中的假定作用。在这项病例对照研究中，通过巢式PCR策略评估了CRC患者（病例n = 45）和无癌受试者的正常结肠黏膜（对照n = 36）中HPV感染的患病率。对HPV阳性组织进行HPV-16基因分型，并通过E2检测确定HPV-16基因组的物理状态。在45例CRC病例中有19例（42.2%）检测到HPV（平均年龄61.1±10.7岁，24例男性），在36例对照中有1例（2.8%）检测到HPV（平均年龄60.9±9.6岁，24例男性），OR = 25.58（95%CI 3.21至203.49）。在63.2%的HPV阳性结直肠肿瘤中检测到HPV-16；在所有HPV-16阳性病例中均观察到基因组整合。这是首份显示加勒比西班牙裔结直肠肿瘤中HPV感染患病率很高的报告。尽管有证据表明HPV整合到宿主基因组中，但仍需要进一步进行机制分析，以研究HPV癌蛋白的表达以及这些癌蛋白在结直肠癌发生中的假定作用。
