National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea.
Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea.
Mar Drugs. 2022 Jul 23;20(8):471. doi: 10.3390/md20080471.
is a brown seaweed, which is abundantly distributed in South Africa. This study investigated an efficient approach using high-performance centrifugal partition chromatography (HPCPC), which has been successfully developed for the isolation and purification of phlorotannins, eckmaxol, and dieckol from the ethyl acetate fraction of (EEM). We evaluated EEM for its inhibitory effect against lipopolysaccharide (LPS)-induced inflammatory responses in zebrafish embryos. The separation of eckmaxol and dieckol from samples of EEM using HPCPC was found to be of high purity and yield under an optimal solvent system composed of n-hexane:ethyl acetate:methanol:water (2:7:3:7, ///). To evaluate the anti-inflammatory efficacy of EEM containing active compounds, zebrafish embryos exposed to LPS were compared with and without EEM treatment for nitric oxide (NO) production, reactive oxygen species (ROS) generation, and cell death two days after fertilization. These evaluations indicate that EEM alleviated inflammation by inhibiting cell death, ROS, and NO generation induced by LPS treatment. According to these results, eckmaxol and dieckol isolated from brown seaweed could be considered effective anti-inflammatory agents as pharmaceutical and functional food ingredients.
裙带菜是一种褐藻，在南非广泛分布。本研究采用高效逆流色谱（HPCPC）对裙带菜乙酸乙酯提取物（EEM）中的多酚、 Eckmaxol 和 Dieckol 进行了分离和纯化。通过 LPS 诱导斑马鱼胚胎炎症反应模型，评价了裙带菜提取物（EEM）的抗炎活性。结果表明，在正相高效逆流色谱分离中，当两相溶剂系统组成为正己烷-乙酸乙酯-甲醇-水（2:7:3:7，///）时，Eckmaxol 和 Dieckol 能得到较好的分离。为了评价含活性化合物的 EEM 的抗炎功效，对 LPS 处理的斑马鱼胚胎与用 EEM 处理的斑马鱼胚胎进行了比较，检测 NO 生成、ROS 产生和细胞死亡。结果表明，EEM 通过抑制 LPS 诱导的细胞死亡、ROS 和 NO 生成，从而减轻炎症。这些结果表明，从褐藻中分离得到的 Eckmaxol 和 Dieckol 可作为药物和功能性食品成分的有效抗炎剂。
