Butkeviciute Aurita, Ramanauskiene Kristina, Kurapkiene Vaida, Janulis Valdimaras
Department of Pharmacognosy, Lithuanian University of Health Sciences, Sukileliu Ave. 13, LT-50162 Kaunas, Lithuania.
Department of Clinical Pharmacy, Lithuanian University of Health Sciences, Sukileliu Ave. 13, LT-50162 Kaunas, Lithuania.
Plants (Basel). 2022 Jul 22;11(15):1901. doi: 10.3390/plants11151901.
Phenolic compounds with miscellaneous biological activities are an interesting component in dermatology and cosmetology practices. The aim of our study was to determine the phenolic compounds released from emulsion, emulgel, gel, ointment, and oleogel formulations penetration into human skin layers, both the epidermis and dermis, and estimate their antioxidant activity. The ex vivo penetration study was performed using Bronaugh type flow-through diffusion cells. Penetration studies revealed that, within 24 h, the chlorogenic acid released from the oleogel penetrated into skin layers to a depth of 2.0 ± 0.1 µg/mL in the epidermis and 1.5 ± 0.07 µg/mL in the dermis. The oleogel-released complex of phenolic compounds penetrating into epidermis showed the strongest DPPH free radical scavenging activity (281.8 ± 14.1 µM TE/L). The study estimated a strong positive correlation (r = 0.729) between the amount of quercetin penetrated into epidermis and the antioxidant activity detected in the epidermis extract. Plant based phenolic compounds demonstrated antioxidant activity and showed great permeability properties through the skin.
具有多种生物活性的酚类化合物是皮肤科和美容实践中一种有趣的成分。我们研究的目的是确定从乳液、乳胶、凝胶、软膏和油凝胶配方中释放的酚类化合物渗透到人体皮肤层（包括表皮和真皮）的情况，并评估它们的抗氧化活性。使用布罗瑙型流通扩散池进行了体外渗透研究。渗透研究表明，在24小时内，从油凝胶中释放的绿原酸渗透到皮肤层的深度在表皮中为2.0±0.1μg/mL，在真皮中为1.5±0.07μg/mL。渗透到表皮中的油凝胶释放的酚类化合物复合物表现出最强的DPPH自由基清除活性（281.8±14.1μM TE/L）。该研究估计，渗透到表皮中的槲皮素量与在表皮提取物中检测到的抗氧化活性之间存在很强的正相关（r = 0.729）。植物来源的酚类化合物表现出抗氧化活性，并显示出通过皮肤的良好渗透性。
