College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Int J Mol Sci. 2022 Jul 25;23(15):8172. doi: 10.3390/ijms23158172.
A regulator of chromosome condensation 1 (RCC1) family protein has been functionally characterized to be involved in various cellular processes. In this study, one gene named was cloned from the full-length cDNA library of . The open reading frame (ORF) of was 1440 bp, and it encoded 479 amino acids with a calculated molecular mass of 51.65 kDa. Multiple amino acid sequence alignments showed that SaRCC1 had high identity with other plant RCC1s, and the phylogenetic analysis indicated that SaRCC1 had a closer affinity to RCC1 family protein (ZmRCC1). gene was induced under salt stress conditions, and its encoded protein was located in peroxisome. In order to further investigate the function of , transgenic plants ectopically both sense-overexpressing and antisense-overexpressing were generated. -overexpressing lines exhibited an increased salt and ABA hypersensitivity and reduced resistance to salinity stress. On the other hand, the transcripts of some stress-responsive genes in the transgenic plants were affected in response to salinity stress. Our results provide evidence for the involvement of , negatively regulating salt stress responses by affecting stress-related gene expression in .
一种着丝粒蛋白 1（RCC1）家族蛋白调节剂的功能已被鉴定参与各种细胞过程。在这项研究中，从 全长 cDNA 文库中克隆出一个名为 的基因。 的开放阅读框（ORF）为 1440bp，编码 479 个氨基酸，计算分子量为 51.65kDa。多种氨基酸序列比对表明，SaRCC1 与其他植物 RCC1 具有高度的同一性，系统进化分析表明，SaRCC1 与 RCC1 家族蛋白（ZmRCC1）具有更密切的亲缘关系。 在盐胁迫条件下诱导表达，其编码的蛋白定位于过氧化物酶体。为了进一步研究 基因的功能，通过反义过表达和正义过表达分别生成了拟南芥的转基因植株。-过表达系表现出对盐和 ABA 的超敏感性增加，对盐胁迫的抗性降低。另一方面，转 基因植物中一些与胁迫相关基因的转录本在响应盐胁迫时受到影响。我们的结果为 基因参与负调控盐胁迫反应提供了证据，通过影响胁迫相关基因的表达来调节盐胁迫反应。
