, a Regulator of Chromosome Condensation 1 (RCC1) Family Protein Gene from , Negatively Regulates Salinity Stress Tolerance in Transgenic .

A regulator of chromosome condensation 1 (RCC1) family protein has been functionally characterized to be involved in various cellular processes. In this study, one gene named was cloned from the full-length cDNA library of . The open reading frame (ORF) of was 1440 bp, and it encoded 479 amino acids with a calculated molecular mass of 51.65 kDa. Multiple amino acid sequence alignments showed that SaRCC1 had high identity with other plant RCC1s, and the phylogenetic analysis indicated that SaRCC1 had a closer affinity to RCC1 family protein (ZmRCC1). gene was induced under salt stress conditions, and its encoded protein was located in peroxisome. In order to further investigate the function of , transgenic plants ectopically both sense-overexpressing and antisense-overexpressing were generated. -overexpressing lines exhibited an increased salt and ABA hypersensitivity and reduced resistance to salinity stress. On the other hand, the transcripts of some stress-responsive genes in the transgenic plants were affected in response to salinity stress. Our results provide evidence for the involvement of , negatively regulating salt stress responses by affecting stress-related gene expression in .