微流控电阻脉冲传感技术对天然 SARS-CoV-2 病毒颗粒的单颗粒检测。

Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, H-1117 Budapest, Hungary.

National Laboratory of Virology, Szentágothai Research Centre, University of Pécs, Ifjúság Útja 20, H-7624 Pécs, Hungary.

Colloids Surf B Biointerfaces. 2022 Oct;218:112716. doi: 10.1016/j.colsurfb.2022.112716. Epub 2022 Jul 22.

Microfluidic resistive pulse sensing (MRPS) was used to determine the size -distribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on detecting nearly 30,000 single virions. However, the ultrastructure of SARS-CoV-2 is thoroughly described, but ensemble properties of SARS-CoV-2, e.g., its particle size distribution, are sparsely reported. According to the MRPS results, the size distribution of SARS-CoV-2 follows a log-normal function with a mean value of 85.1 nm, which corresponds to an approximate diameter of the viral envelope. This result also confirms the low number (< 50) of spike proteins on the surface of the virions.

微流控电阻脉冲感应 (MRPS) 技术被用于通过检测近 30000 个单个病毒粒子来确定严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的大小分布。然而，SARS-CoV-2 的超微结构被详细描述，但 SARS-CoV-2 的总体性质，例如其粒径分布，报道较少。根据 MRPS 结果，SARS-CoV-2 的尺寸分布遵循对数正态函数，平均值为 85.1nm，对应于病毒包膜的近似直径。这一结果也证实了病毒粒子表面刺突蛋白的数量较少（<50）。