Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia; Winship Cancer Institute, Emory University, Atlanta, Georgia.
Rollins School of Public Health, Emory University, Atlanta, Georgia.
Pain Manag Nurs. 2023 Feb;24(1):35-43. doi: 10.1016/j.pmn.2022.06.002. Epub 2022 Jul 28.
The gut microbiome seems to play a role in migraines through increasing intestinal epithelial permeability and pro-inflammatory processes. The associations between the gut microbiome and migraines are uncertain in children.
The purpose of this quantitative study was to examine the associations between the gut microbiome and migraines in children aged 7-18 years from the American Gut Project (AGP).
A cohort of children aged 7-18 years from the AGP was analyzed. 16S rRNA V4 gene sequences for the gut microbiome, migraines, and demographics were obtained from the AGP Public Repository. After quality control of 16S rRNA gene sequences, α-diversity (Shannon, Faith's_PD, and evenness) and β-diversity metrics (Bray-Curtis and weighted-UniFrac distances), taxonomy, and abundance analyses were implemented using QIIME 2.
In total, 381 children (341 without migraines; 40 with professional or self-diagnosed migraines) were analyzed with a mean age of 11.5 years. Compared with those without migraines, children with migraines showed lower estimates in Shannon and Faith's_PD (p < .01). Both Bray-Curtis and weighted-UniFrac distances displayed the gut microbial dissimilarities between these two groups (p = .001). Children with migraines had higher abundances in genus of phylum Bacteroidetes (Bacteroides, Parabacteroides, Odoribacter), Actinobacteria (Eggerthella, Varibaculum), Firmicutes (SMB53, Lachnospira, Dorea, Veillonella, Anaerotruncus, Butyricicoccus, Coprobacillus, Eubacterium), and Proteobacteria (Sutterella) than children without migraines.
Associations of the gut microbiome diversity and abundances with migraines in children indicated potential biological mechanisms of migraines. Future work needs to confirm our findings in children.
肠道微生物群似乎通过增加肠道上皮通透性和促炎过程在偏头痛中发挥作用。肠道微生物群与偏头痛之间的关联在儿童中尚不确定。
本定量研究的目的是在美国肠道计划（AGP）中检查 7-18 岁儿童的肠道微生物群与偏头痛之间的关联。
对 AGP 中的 7-18 岁儿童队列进行了分析。从 AGP 公共存储库中获得了肠道微生物群、偏头痛和人口统计学的 16S rRNA V4 基因序列。在对 16S rRNA 基因序列进行质量控制后，使用 QIIME 2 进行了 α 多样性（香农、信仰的 PD 和均匀度）和 β 多样性指标（Bray-Curtis 和加权 UniFrac 距离）、分类学和丰度分析。
总共分析了 381 名儿童（341 名无偏头痛；40 名有专业或自我诊断的偏头痛），平均年龄为 11.5 岁。与无偏头痛的儿童相比，偏头痛儿童的香农和信仰的 PD 估计值较低（p<0.01）。Bray-Curtis 和加权 UniFrac 距离均显示出两组之间肠道微生物的差异（p=0.001）。偏头痛儿童的厚壁菌门（拟杆菌属、副拟杆菌属、恶臭杆菌属）、放线菌门（埃格特氏菌属、瓦氏菌属）、Firmicutes（SMB53、lachnospira、Dorea、Villonella、Anaerotruncus、Butyricicoccus、Coprobacillus、Eubacterium）和 Proteobacteria（Sutterella）属的丰度高于无偏头痛的儿童。
肠道微生物群多样性和丰度与儿童偏头痛之间的关联表明了偏头痛的潜在生物学机制。未来的工作需要在儿童中证实我们的发现。
