BGI-Shenzhen, Shenzhen, China.
Suzhou Institute of Systems Medicine, Suzhou, Jiangsu, China.
Clin Transl Med. 2022 Aug;12(8):e886. doi: 10.1002/ctm2.886.
The exact animal origin of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains obscure and understanding its host range is vital for preventing interspecies transmission.
Herein, we applied single-cell sequencing to multiple tissues of 20 species (30 data sets) and integrated them with public resources (45 data sets covering 26 species) to expand the virus receptor distribution investigation. While the binding affinity between virus and receptor is essential for viral infectivity, understanding the receptor distribution could predict the permissive organs and tissues when infection occurs.
Based on the transcriptomic data, the expression profiles of receptor or associated entry factors for viruses capable of causing respiratory, blood, and brain diseases were described in detail. Conserved cellular connectomes and regulomes were also identified, revealing fundamental cell-cell and gene-gene cross-talks from reptiles to humans.
Overall, our study provides a resource of the single-cell atlas of the animal kingdom which could help to identify the potential host range and tissue tropism of viruses and reveal the host-virus co-evolution.
严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)的确切动物来源仍不清楚，了解其宿主范围对于防止种间传播至关重要。
本文通过单细胞测序技术，对 20 个物种的多种组织（30 个数据集）进行了分析，并整合了公共资源（涵盖 26 个物种的 45 个数据集），以扩大对病毒受体分布的调查。虽然病毒与受体之间的结合亲和力对于病毒的感染力至关重要，但了解受体的分布可以预测感染发生时允许的器官和组织。
基于转录组数据，详细描述了能够引起呼吸道、血液和脑部疾病的病毒的受体或相关进入因子的表达谱。还鉴定了保守的细胞连接组和调控组，揭示了从爬行动物到人基本的细胞间和基因间相互作用。
总的来说，我们的研究提供了动物王国的单细胞图谱资源，有助于识别病毒的潜在宿主范围和组织嗜性，并揭示宿主-病毒的共同进化。
