Centro Universitário Sagrado Coração, Unisagrado , Curso de Odontologia, Bauru , SP , Brasil .
Universidade de São Paulo , Faculdade de Odontologia de Bauru , Departamento de Dentística, Endodontia e Materiais Odontológicos , Bauru , SP , Brasil .
J Appl Oral Sci. 2022 Aug 1;30:e20220108. doi: 10.1590/1678-7757-2022-0108. eCollection 2022.
Filling materials should be restricted to the root canal space. However, sometimes it is impossible to control the apical extrusion, in this case, the fate of the filling material and the result of the treatment will depend on its physicochemical properties and biocompatibility. To evaluate the tissue response and bone repair capacity of endodontic sealers that were implanted in the calvaria of Wistar rats, forming the groups (n=16): AH Plus and Sealer Plus, compared to the clot group.
On days 30 and 60, the animals were euthanized, the calvaria was removed and processed for hematoxylin-eosin, immunohistochemistry for collagen type I, Picrosirus red and microtomographic analysis. Data were subjected to ANOVA and Tuckey tests (p<0.05).
At 30 days, all groups showed an intense inflammatory reaction (p>0.05). At 60 days, the AH Plus and Sealer Plus maintained an intense inflammatory infiltrate compared to the clot group (p<0.05). We observed immunopositive areas for type I collagen in all groups at 30 days and 60 days (p>0.05). We observed more red collagen fibers for the Sealer Plus compared to the clot group at 30 days (p<0.05). Considering the total fibers, the clot group at 30 days compared to 60 days after surgery showed an increase in the amount of matrix (p<0.05). There were no statistical differences between groups for green and yellow fibers (p>0.05). Regarding morphometric parameters, at 30 days, the newly formed bone volume and number of bone trabeculae were higher in the groups with sealers compared to the clot group (p<0.05). At 60 days, AH Plus and Sealer Plus showed greater bone neoformation compared to the clot group (p<0.05).
Despite AH Plus and Sealer Plus induced an intense inflammatory reaction, they can be considered biocompatible materials, since they allowed bone repair.
填充材料应限制在根管空间内。然而，有时控制根尖挤出是不可能的，在这种情况下，填充材料的命运和治疗结果将取决于其物理化学性质和生物相容性。为了评估在 Wistar 大鼠颅骨中植入的根管封闭剂的组织反应和骨修复能力，将其分为（n=16）：AH Plus 和 Sealer Plus 组，并与血凝块组进行比较。
在第 30 天和第 60 天，处死动物，取出颅骨并进行苏木精-伊红、I 型胶原免疫组织化学、Picrosirus 红和微断层分析。数据采用方差分析和 Tukey 检验（p<0.05）。
在第 30 天，所有组均表现出强烈的炎症反应（p>0.05）。在第 60 天，与血凝块组相比，AH Plus 和 Sealer Plus 仍保持强烈的炎症浸润（p<0.05）。在第 30 天和第 60 天，所有组均观察到 I 型胶原免疫阳性区域（p>0.05）。与血凝块组相比，第 30 天 Sealer Plus 观察到更多的红色胶原纤维（p<0.05）。考虑到总纤维，与术后第 30 天相比，血凝块组在第 60 天术后的基质量增加（p<0.05）。各组之间的绿色和黄色纤维无统计学差异（p>0.05）。关于形态计量学参数，在第 30 天，与血凝块组相比，含封闭剂组的新生骨体积和骨小梁数量较高（p<0.05）。在第 60 天，AH Plus 和 Sealer Plus 与血凝块组相比，显示出更大的新骨形成（p<0.05）。
尽管 AH Plus 和 Sealer Plus 引起强烈的炎症反应，但它们可被视为生物相容材料，因为它们允许骨修复。
