Department of Natural and Applied Sciences, Faculty of Health and Applied Sciences, Namibia University of Science and Technology, Windhoek, Namibia.
PLoS One. 2022 Aug 4;17(8):e0272457. doi: 10.1371/journal.pone.0272457. eCollection 2022.
Nigella sativa is one of the medicinal plant species that gained popularity for a wide range of medicinal applications due to its seeds which are rich in phytoconstituents. Continuous scientific investigations on N. sativa seeds are needed to better understand its many medicinal potentials. This will also form a composition-based foundation that support several old and/or new case beneficial histories of its seeds. In this study, the antimicrobial activity of N. sativa seeds was phytochemically characterized and evaluated. Different extracts of N. sativa seeds were obtained by maceration and soxhlet extraction methods using different extraction solvents. The obtained extracts were tested using UV-Vis, FTIR, TLC, and GC-MS techniques. Antimicrobial analysis against pathogenic bacterial strains (E. coli, P. aeruginosa, S. aureus and B. subtilis) was carried out by disc diffusion method using different preparations of N. sativa seeds. The screening analysis revealed the presence of all the tested phytochemicals. FT-IR analysis of N. sativa seeds oil extracted with absolute ethanol revealed functional groups that are associated with active ingredients of medicinal value. The GC-MS chromatograms revealed different chemical constituents whose known bioactivities and/or applications are essential in the management of life-threatening infections. Different extracts of N. sativa seeds showed antimicrobial activity with different efficacy against the tested pathogenic bacterial strains. Therefore, this study shows that extracts of N. sativa seeds contain a variety of chemical components and functional groups linked to their antimicrobial properties, and they might be natural precursors of nutraceuticals.
黑种草是一种药用植物，由于其种子富含植物成分，因此在广泛的药用应用中广受欢迎。需要对黑种草种子进行持续的科学研究，以更好地了解其许多药用潜力。这也将形成一个基于成分的基础，支持其种子的几个古老和/或新的有益案例历史。在这项研究中，对黑种草种子的抗菌活性进行了植物化学表征和评估。通过浸渍和索氏提取方法使用不同的提取溶剂从黑种草种子中获得不同的提取物。使用 UV-Vis、FTIR、TLC 和 GC-MS 技术测试了获得的提取物。通过使用不同制备的黑种草种子的圆盘扩散法对致病菌株（大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和枯草芽孢杆菌）进行了抗菌分析。筛选分析显示存在所有测试的植物化学物质。用无水乙醇提取的黑种草种子油的 FT-IR 分析显示出与具有药用价值的活性成分相关的功能基团。GC-MS 色谱图显示了不同的化学成分，其已知的生物活性和/或应用在危及生命的感染的管理中至关重要。黑种草种子的不同提取物对测试的致病菌表现出不同疗效的抗菌活性。因此，这项研究表明，黑种草种子的提取物含有多种与抗菌特性相关的化学成分和功能基团，它们可能是天然营养保健品的前体。
