Orshiso Temesgen Achamo, Zereffa Enyew Amare, Murthy H C Ananda, Demissie Taye B, Pardeshi Onkar, Avhad Lata S, Ghotekar Suresh
Department of Applied Chemistry, School of Applied Natural Sciences, Adama Science and Technology University, P.O. Box 1888, Adama 1888, Ethiopia.
Department of Prosthodontics, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Science (SIMAT), Saveetha University, Chennai 600077, Tamil Nadu, India.
ACS Omega. 2023 Oct 24;8(44):41039-41053. doi: 10.1021/acsomega.3c01814. eCollection 2023 Nov 7.
Currently, plant extract-mediated synthesized metal oxide nanoparticles (MO NPs) have played a substantial role in biological applications. Hence, this study focused on the eco-benign one-pot synthesis of bimetallic ZnO-CuO nanoparticles (ZC NPs) using the leaf extract of (LEAA) and evaluations of their anticancer, antioxidant, and molecular binding efficacy. The optical absorption peak at 380 nm from UV-visible (UV-vis) analysis revealed the formation of ZC NPs. X-ray diffraction (XRD) results revealed the fabrication of mixed-phase crystals with hexagonal and monoclinic structures of ZC NPs with an average crystallite size of 14 nm. Moreover, the biosynthesis of ZC NPs with a spherical morphology and an average particle size of 13.09 nm was confirmed by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) results. Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA/DTA) spectroscopy confirmed the involvement of functional groups from LEAA during the synthesis of ZC NPs. ZC NPs have exhibited the ferric ion reducing power (FRAP) with an absorbance of 1.826 ± 0.00 at 200 μg/mL and DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity of 95.71 ± 0.02% at 200 μg/mL with an IC50 value of 3.28 μg/mL. Moreover, ZC NPs had shown a promising in vitro anticancer activity of 89.20 ± 0.038 at 500 μg/mL with an IC50 value of 33.12 μg/mL against breast cancer (MCF-7) cell lines. Likewise, ZC NPs have shown strong binding affinity (-8.50 kcal/mol) against estrogen receptor α (ERα) in molecular docking simulations. These findings suggested that the biosynthesized ZC NPs could be used as promising antioxidant and anticancer drug candidates, particularly for breast cancer ailments. However, the cytotoxicity test will be recommended to ensure further use of ZC NPs.
目前，植物提取物介导合成的金属氧化物纳米颗粒（MO NPs）在生物应用中发挥了重要作用。因此，本研究聚焦于使用（LEAA）叶提取物进行双金属ZnO-CuO纳米颗粒（ZC NPs）的生态友好型一锅法合成，并评估其抗癌、抗氧化和分子结合功效。紫外-可见（UV-vis）分析在380 nm处的光吸收峰揭示了ZC NPs的形成。X射线衍射（XRD）结果显示，ZC NPs为具有六方和单斜结构的混合相晶体，平均晶粒尺寸为14 nm。此外，扫描电子显微镜（SEM）、能量色散X射线（EDX）和透射电子显微镜（TEM）结果证实了ZC NPs的球形形态和平均粒径为13.09 nm的生物合成。傅里叶变换红外（FTIR）和热重分析（TGA/DTA）光谱证实了LEAA中的官能团在ZC NPs合成过程中的参与。ZC NPs在200 μg/mL时表现出铁离子还原能力（FRAP），吸光度为1.826±0.00，在200 μg/mL时DPPH（2,2-二苯基-1-苦基肼水合物）自由基清除活性为95.71±0.02%，IC50值为3.28 μg/mL。此外，ZC NPs在500 μg/mL时对乳腺癌（MCF-7）细胞系表现出89.20±0.038的有前景的体外抗癌活性，IC50值为33.12 μg/mL。同样，在分子对接模拟中，ZC NPs对雌激素受体α（ERα）显示出很强的结合亲和力（-8.50 kcal/mol）。这些发现表明，生物合成的ZC NPs可用作有前景的抗氧化剂和抗癌药物候选物，特别是用于乳腺癌疾病。然而，建议进行细胞毒性测试以确保ZC NPs的进一步使用。
