Ripa Farhana Alam, Hossain Md Jamal, Nesa Mst Luthfun, Zahan Miss Sharmin, Mitra Saikat, Rashid Mohammad A, Roy Arpita, Alghamdi Saad, Almehmadi Mazen, Abdulaziz Osama
Department of Pharmacy, Brac University, 41-Pacific Tower, Mohakhali, Dhaka 1212, Bangladesh.
Department of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka 1205, Bangladesh.
Evid Based Complement Alternat Med. 2022 Jul 18;2022:2594127. doi: 10.1155/2022/2594127. eCollection 2022.
Medicinal plants have been crucial in treating various chronic ailments since ancient times. The objective of this study was to evaluate in vitro pharmacological properties of petroleum ether, chloroform, and ethyl acetate soluble fractions of ethanolic extract (leaf, bark, and root) of Buch. Ham., including the phytochemical screening of the plant. Thrombolytic and antiarthritic properties were assessed through the clot lysis and protein denaturation experimental method, correspondingly. Anthelmintic and insecticidal activities were studied against and , respectively. The phytochemical analysis exhibited numerous active phytochemicals in different solvent fractions. In thrombolytic investigation, among all crude extracts, ethanolic leaf extract showed the highest 33.12 ± 7.52% clot lysis as compared to standard streptokinase (67.77 ± 9.78%). In antiarthritic assay, all the tested samples exhibited noteworthy protein denaturation in dose-dependent manner (100-500 g/mL), whereas the utmost percentage inhibition was noticed for chloroform extract of roots (63.28 ± 5.96% at 500 g/mL). All crude extracts exhibited a significant anthelmintic activity in different concentrations (25-75 mg/mL) and revealed paralysis and death of earthworms in comparison with albendazole; ethanolic extract of the bark was found to be more potent at the highest dose. For the insecticidal test, ethanolic extract of the leaf showed the utmost mortality rate (73%). The outcomes of the investigation confirmed the potential thrombolytic, antiarthritic, anthelmintic, and insecticidal activities of the different extracts of , and hence, advanced studies on the isolation and identification of active phytocompounds are highly needed for new drug development.
自古以来，药用植物在治疗各种慢性疾病方面发挥了关键作用。本研究的目的是评估布氏（Buch. Ham.）乙醇提取物（叶、树皮和根）的石油醚、氯仿和乙酸乙酯可溶部分的体外药理特性，包括对该植物进行植物化学筛选。分别通过凝块溶解和蛋白质变性实验方法评估溶栓和抗关节炎特性。分别针对[具体寄生虫名称1]和[具体昆虫名称1]研究驱虫和杀虫活性。植物化学分析表明，不同溶剂部分含有多种活性植物化学物质。在溶栓研究中，与标准链激酶（67.77±9.78%）相比，在所有粗提物中，乙醇叶提取物的凝块溶解率最高，为33.12±7.52%。在抗关节炎试验中，所有测试样品在剂量依赖性方式（100 - 500μg/mL）下均表现出显著的蛋白质变性，而根部氯仿提取物的抑制率最高（500μg/mL时为63.28±5.96%）。所有粗提物在不同浓度（25 - 75mg/mL）下均表现出显著的驱虫活性，与阿苯达唑相比，蚯蚓出现麻痹和死亡；树皮乙醇提取物在最高剂量下更有效。在杀虫试验中，叶乙醇提取物的死亡率最高（73%）。研究结果证实了布氏不同提取物具有潜在的溶栓、抗关节炎、驱虫和杀虫活性，因此，为开发新药，迫切需要对活性植物化合物进行分离和鉴定的进一步研究。
