Department of Internal Medicine and Oncology, Semmelweis University, Budapest, Hungary.
Molecular Medicine Research Group, Eötvös Loránd Research Network, Budapest, Hungary.
Pathol Oncol Res. 2022 Jul 14;28:1610342. doi: 10.3389/pore.2022.1610342. eCollection 2022.
In recent years, the evolution of the molecular biological technical background led to the widespread application of single-cell sequencing, a versatile tool particularly useful in the investigation of tumor heterogeneity. Even 10 years ago the comprehensive characterization of colorectal cancers by The Cancer Genome Atlas was based on measurements of bulk samples. Nowadays, with single-cell approaches, tumor heterogeneity, the tumor microenvironment, and the interplay between tumor cells and their surroundings can be described in unprecedented detail. In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers.
近年来，分子生物学技术背景的发展使得单细胞测序得到了广泛应用，这是一种非常有用的工具，尤其适用于研究肿瘤异质性。即使在 10 年前，癌症基因组图谱（The Cancer Genome Atlas）对结直肠癌的全面表征也是基于对大量样本的测量。如今，通过单细胞方法，可以以前所未有的细节描述肿瘤异质性、肿瘤微环境以及肿瘤细胞与其周围环境之间的相互作用。在这篇综述文章中，我们旨在通过展示肿瘤异质性和传统研究方法的局限性来强调单细胞分析的重要性，接着概述从样本分离到扩增、测序和生物信息学分析的整个单细胞分析工作流程，并回顾关于结直肠癌单细胞分析的最新文献。
