Department of Ultrasonic Imaging, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Department of Gastroenterology, Minhang Hospital, Fudan University, 170 Xinsong Road, Shanghai 201199, China.
Comput Math Methods Med. 2022 Jul 28;2022:5385456. doi: 10.1155/2022/5385456. eCollection 2022.
Although the emerging of immunotherapy conferred a new landscape of gastric cancer (GC) treatment, its response rate was of significant individual differences. Insight into GC immune microenviroment may contribute to breaking the dilemma. To this end, the enrichment score of NF-B signaling pathway was calculated in each GC sample from The Cancer Genome Atlas (TCGA) via ssGSEA algorithm, and its association with immune infiltration was estimated. Based on NF-B-related genes, a risk score was established and its involvement in immune infiltration, tumor mutational burden (TMB), and N6-methyladenosine (M6A) modification was analyzed in GC. The results showed that NF-B signaling pathway promoted the infiltration of immune cells in GC. In addition, GC samples were divided into low- and high-risk groups according to a seven-gene (, , , , , , and ) risk score. Although the high-risk group displayed high immune infiltration and high expression of M6A regulatory genes, it remains in an immunosuppressive microenviroment and whereby suffers a poorer outcome. Of note, most of hub genes were related to immune infiltration and could serve as an independent prognostic biomarker. Conclusively, our study emphasized the crucial role of NF-B signaling pathway in GC immune microenviroment and provided several candidate genes that may participate in immune infiltration.
尽管免疫疗法的出现为胃癌（GC）治疗带来了新的局面，但它的反应率存在显著的个体差异。深入了解 GC 的免疫微环境可能有助于打破这一困境。为此，我们通过 ssGSEA 算法计算了来自癌症基因组图谱（TCGA）的每个 GC 样本中 NF-B 信号通路的富集评分，并评估了其与免疫浸润的相关性。基于 NF-B 相关基因，建立了风险评分，并分析了其在 GC 中的免疫浸润、肿瘤突变负担（TMB）和 N6-甲基腺苷（M6A）修饰中的作用。结果表明，NF-B 信号通路促进了 GC 中免疫细胞的浸润。此外，根据一个包含七个基因（,,,,,, 和 ）的风险评分，GC 样本被分为低风险组和高风险组。尽管高风险组显示出高免疫浸润和高 M6A 调节基因表达，但它仍处于免疫抑制微环境中，因此预后较差。值得注意的是，大多数关键基因都与免疫浸润有关，可作为独立的预后生物标志物。总之，本研究强调了 NF-B 信号通路在 GC 免疫微环境中的关键作用，并提供了几个可能参与免疫浸润的候选基因。
