Jiao Chunyan, Wei Mengke, Fan Honghong, Song Cheng, Wang Zhanjun, Cai Yongping, Jin Qing
School of Life Sciences, Anhui Agricultural University, Hefei, China.
College of Life Sciences, Hefei Normal University, Hefei, China.
Front Plant Sci. 2022 Jul 22;13:941231. doi: 10.3389/fpls.2022.941231. eCollection 2022.
is both a traditional herbal medicine and a plant of high ornamental and medicinal value. Alkaloids, especially terpenoid indole alkaloids (TIAs), with pharmacological activities are present in the tissues of . A number of genes involved in alkaloid biosynthetic pathways have been identified. However, the regulatory mechanisms underlying the precursor and methyl jasmonate (MeJA)-induced accumulation of alkaloids in are poorly understood. In this study, we collected protocorm-like bodies (PLBs) and treated them with TIA precursors (tryptophan and secologanin) and MeJA for 0 (T0), 4 (T4) and 24 h (T24); we also established control samples (C4 and C24). Then, we measured the total alkaloid content of the PLBs and performed transcriptome sequencing using the Illumina HiSeq 2,500 system. The total alkaloid content increased significantly after 4 h of treatment. Go and KEGG analysis suggested that genes from the TIA, isoquinoline alkaloid, tropane alkaloid and jasmonate (JA) biosynthetic pathways were significantly enriched. Weighted gene coexpression network analysis (WGCNA) uncovered brown module related to alkaloid content. Six and seven genes related to alkaloid and JA bisosynthetic pathways, respectively, might encode the key enzymes involved in alkaloid biosynthesis of . Moreover, 13 transcription factors (TFs), which mostly belong to AP2/ERF, WRKY, and MYB gene families, were predicted to regulate alkaloid biosynthesis. Our data provide insight for studying the regulatory mechanism underlying TIA precursor and MeJA-induced accumulation of three types of alkaloids in .
既是一种传统草药，又是一种具有高观赏和药用价值的植物。生物碱，尤其是具有药理活性的萜类吲哚生物碱（TIAs），存在于其组织中。已经鉴定出许多参与生物碱生物合成途径的基因。然而，前体和茉莉酸甲酯（MeJA）诱导生物碱在其中积累的调控机制尚不清楚。在本研究中，我们收集了原球茎状体（PLBs），并用TIA前体（色氨酸和裂环马钱子苷）和MeJA处理0（T0）、4（T4）和24小时（T24）；我们还建立了对照样品（C4和C24）。然后，我们测量了PLBs的总生物碱含量，并使用Illumina HiSeq 2500系统进行转录组测序。处理4小时后，总生物碱含量显著增加。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，来自TIA、异喹啉生物碱、托烷生物碱和茉莉酸（JA）生物合成途径的基因显著富集。加权基因共表达网络分析（WGCNA）揭示了与生物碱含量相关的棕色模块。分别有六个和七个与生物碱和JA生物合成途径相关的基因可能编码参与生物碱生物合成的关键酶。此外，预测有13个转录因子（TFs），其中大部分属于AP2/ERF、WRKY和MYB基因家族，可调节生物碱的生物合成。我们的数据为研究TIA前体和MeJA诱导三种生物碱在其中积累的调控机制提供了见解。
