Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005477.
Two Gram-stain-negative, aerobic and yellow-pigmented bacterial strains, designated K20-16 and MSW2, were isolated from a marine red alga ( species) and seawater, respectively. Both strains were oxidase-positive, weakly catalase-positive and non-flagellated rods with gliding motility. Menaquinone-6 was detected as the sole isoprenoid quinone in both strains. Iso-C, iso-C 3-OH, iso-C G, C 6 and summed feature 3 (comprising C 7 and/or C 6) were identified in both strains as major fatty acids. Phosphatidylethanolamine was not identified in strain K20-16, but it was identified in strain MSW2. The genomic DNA G+C contents of strains K20-16 and MSW2 were 30.5 and 30.7 %, respectively. Strains K20-16 and MSW2 shared 99.7% 16S rRNA gene sequence similarity, 97.7% average nucleotide identity (ANI), and 80.5% digital DNA-DNA hybridization (DDH) value, indicating that they are the same species. Phylogenetic analyses based on 16S rRNA gene and 92 concatenated core protein sequences revealed that strains K20-16 and MSW2 formed a phylogenic lineage within the genus and were most closely related to LPB0136 with 98.3 and 98.0% 16S rRNA gene sequence similarities, respectively. ANI and digital DDH values between strains K20-16 and MSW2 and other type strains were less than 91.4 and 43.1 %, respectively. Based on the phenotypic, chemotaxonomic and molecular features, strains K20-16 and MSW2 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K20-16 (=KACC 22 342=JCM 35 023).
两株革兰氏阴性、需氧和黄色着色的细菌菌株，分别命名为 K20-16 和 MSW2，分别从海洋红藻（种）和海水中分离得到。两株菌均为氧化酶阳性、弱过氧化氢酶阳性、无鞭毛的滑行菌。两种菌都检测到唯一的异戊烯醌类为menaquinone-6。两种菌的主要脂肪酸均为iso-C、iso-C 3-OH、iso-C G、C 6 和总和特征 3（包含 C 7 和/或 C 6）。K20-16 菌株中未鉴定出磷脂酰乙醇胺，但在 MSW2 菌株中鉴定出。菌株 K20-16 和 MSW2 的基因组 DNA G+C 含量分别为 30.5%和 30.7%。K20-16 菌株和 MSW2 菌株的 16S rRNA 基因序列相似性为 99.7%，平均核苷酸同一性（ANI）为 97.7%，数字 DNA-DNA 杂交（DDH）值为 80.5%，表明它们是同一物种。基于 16S rRNA 基因和 92 个串联核心蛋白序列的系统发育分析表明，K20-16 菌株和 MSW2 菌株在属内形成一个分支，并与 LPB0136 菌株具有最高的亲缘关系，其 16S rRNA 基因序列相似性分别为 98.3%和 98.0%。K20-16 菌株和 MSW2 菌株与其他模式菌株的 ANI 和数字 DDH 值均小于 91.4%和 43.1%。根据表型、化学分类和分子特征，K20-16 菌株和 MSW2 菌株代表了属的一个新种，建议将其命名为 sp. nov.。模式菌株为 K20-16（=KACC 22342=JCM 35023）。
