Hollins B, Noe B, Henderson J M
Clin Chem. 1987 Jun;33(6):765-8.
With this fluorometric method for measuring indocyanine green (ICG) in biological fluids, the limit of detection is an order of magnitude lower than for the traditional spectrophotometric procedure. The excitation and emission maxima are 780 and 810 nm, respectively. Agreement was excellent (r = 0.998) between direct results by this method and those by a liquid-chromatographic procedure with spectrophotometric detection. ICG breaks down in aqueous solution; the degradation products can be detected with liquid-chromatographic/spectrophotometric methods, but because the metabolites are not fluorescent, they do not interfere in the method present here. The increased specificity and sensitivity of this method should permit much more complete analysis of the kinetics of ICG disposition.
采用这种荧光法测量生物体液中的吲哚菁绿（ICG），其检测限比传统分光光度法低一个数量级。激发和发射最大值分别为780和810 nm。该方法的直接结果与采用分光光度检测的液相色谱法的结果之间具有极好的一致性（r = 0.998）。ICG在水溶液中会分解；其降解产物可用液相色谱/分光光度法检测，但由于这些代谢产物不具有荧光性，因此不会干扰本文所介绍的方法。该方法特异性和灵敏度的提高应能更全面地分析ICG的处置动力学。
