Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2022 Jul 29;23(15):8410. doi: 10.3390/ijms23158410.
Variation in flowering plays a major role in maize photoperiod adaptation during long-term domestication. It is of high value to investigate the genetic basis of maize flowering under a wide range of environmental conditions in order to overcome photoperiod sensitivity or enhance stress tolerance. A recombinant inbred line (RIL) population derived from a cross between Huangzaosi and Mo17, composed of 121 lines and genotyped by 8329 specifically developed markers, was field evaluated in two consecutive years under two planting densities (67,500 and 120,000 plants ha) and two water treatments (normal irrigation and drought stress at the flowering stage). The days to silking (DTS), days to anthesis (DTA), and anthesis to silking interval (ASI) were all evaluated. Within the RIL population, DTS and DTA expanded as planting density and water deficit increased. For DTA, DTS, ASI, and ASI-delay, a total of 22, 17, 21, and 11 QTLs were identified, respectively. More than two significant QTLs were identified in each of the nine chromosomal intervals. Under diverse conditions and locations, six QTLs (quantitative trait locus) for DTS and DTA were discovered in Chr. 8: 118.13-125.31 Mb. Three chromosome regions, Chr. 3: 196.14-199.89 Mb, Chr. 8: 169.02-172.46 Mb, and Chr. 9: 128.12-137.26 Mb, all had QTLs for ASI-delay under normal and stress conditions, suggesting their possible roles in stress tolerance enhancement. These QTL hotspots will promote early-maturing or multiple abiotic stress-tolerant maize breeding, as well as shed light on the development of maize varieties with a broad range of adaptations.
在长期的驯化过程中，开花的变化在玉米对光周期的适应中起着重要作用。研究玉米在广泛环境条件下开花的遗传基础具有很高的价值，以便克服对光周期的敏感性或增强对胁迫的耐受性。由黄早四和 Mo17 杂交衍生的重组自交系 (RIL) 群体，由 121 个系组成，并用专门开发的 8329 个标记进行了基因型分析，在两年内分两个种植密度（67500 和 120000 株/公顷）和两个水分处理（正常灌溉和开花期干旱胁迫）下进行了田间评价。评估了抽丝期（DTS）、开花期（DTA）和开花期到抽丝期的间隔（ASI）。在 RIL 群体中，随着种植密度和水分亏缺的增加，DTS 和 DTA 都增加了。对于 DTA、DTS、ASI 和 ASI 延迟，总共鉴定出 22、17、21 和 11 个 QTL。在九个染色体区间中的每个区间都鉴定出超过两个显著的 QTL。在不同的条件和地点下，在 Chr. 8：118.13-125.31 Mb 中发现了 6 个 DTS 和 DTA 的 QTL。三个染色体区域，Chr. 3：196.14-199.89 Mb、Chr. 8：169.02-172.46 Mb 和 Chr. 9：128.12-137.26 Mb，在正常和胁迫条件下都有 ASI 延迟的 QTL，表明它们可能在增强胁迫耐受性方面的作用。这些 QTL 热点将促进早熟或多种非生物胁迫耐受玉米的选育，并为开发适应广泛的玉米品种提供启示。
