College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
Molecules. 2022 Jul 31;27(15):4903. doi: 10.3390/molecules27154903.
Two new lactones, named Ardisicreolides A-B (-), together with four known flavonoids, Quercetin (), Myricetrin (), Quercitrin (), Tamarixetin 3-O-rhamnoside (), were isolated from the ethyl acetate portion of 70% ethanol extracts of dried leaves from Sims. These compounds were identified from Sims for the first time. The structures of - were elucidated according to 1D and 2D-NMR methods and together with the published literature. All of the isolated compounds were evaluated for in vitro anti-microbial effect against , , , , , and . In addition, compounds - were assessed for anti-inflammatory activity by acting on LPS-induced RAW 264.7 macrophage cells in vitro. The results showed that only compound exhibited moderate antibacterial activity on . Moreover, compounds and were found to significantly inhibit the production of nitric oxide (NO) and reduce the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), and interleukin-10 (IL-10) in LPS-induced RAW 264.7 macrophage cells. The present data suggest that lactones from the leaves of Sims might be used as a potential source of natural anti-inflammatory agents.
两种新的内酯，命名为 Ardisicreolide A-B（-），与四种已知的黄酮类化合物，槲皮素（），杨梅素（），槲皮苷（），柽柳素 3-O-鼠李糖苷（），从 Sims 干叶的 70%乙醇提取物的乙酸乙酯部分分离得到。这些化合物是首次从 Sims 中分离得到的。根据 1D 和 2D-NMR 方法以及已发表的文献，确定了 - 的结构。所有分离得到的化合物均进行了体外抗微生物活性评估，包括、、、、和。此外，通过在 LPS 诱导的 RAW 264.7 巨噬细胞中体外作用，评估了化合物 - 对炎症的抑制活性。结果表明，只有化合物对具有中等的抗菌活性。此外，发现化合物和显著抑制 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）的产生，并减少肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）和白细胞介素-10（IL-10）的释放。这些数据表明，来自 Sims 叶的内酯可能被用作天然抗炎剂的潜在来源。
