Two New Phenolic Glycosides with Lactone Structural Units from Leaves of Sims with Antibacterial and Anti-Inflammatory Activities.

Two new lactones, named Ardisicreolides A-B (-), together with four known flavonoids, Quercetin (), Myricetrin (), Quercitrin (), Tamarixetin 3-O-rhamnoside (), were isolated from the ethyl acetate portion of 70% ethanol extracts of dried leaves from Sims. These compounds were identified from Sims for the first time. The structures of - were elucidated according to 1D and 2D-NMR methods and together with the published literature. All of the isolated compounds were evaluated for in vitro anti-microbial effect against , , , , , and . In addition, compounds - were assessed for anti-inflammatory activity by acting on LPS-induced RAW 264.7 macrophage cells in vitro. The results showed that only compound exhibited moderate antibacterial activity on . Moreover, compounds and were found to significantly inhibit the production of nitric oxide (NO) and reduce the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), and interleukin-10 (IL-10) in LPS-induced RAW 264.7 macrophage cells. The present data suggest that lactones from the leaves of Sims might be used as a potential source of natural anti-inflammatory agents.