Altavas Patrick Josemaria Dr, Abaya Alfonso Rafael G, Abella Remo Vittorio Thaddeus D, Acosta Danna Lee A, Aguilar Angelica C, Aguinaldo Camille Anne V, Aguirre Katrina Loise L, Amante Catherine Therese C, Amora Karen B, Anarna Glen Aldrix R, Andrada Rafael T, Ang Gere Ganixon T, Angobung Jeram Caezar R, Aquino Angelo V, Arabis Dennielle Ann P, Awitan Hannah Luisa G, Baccay Mary Faith D, Bagsic Chryz Angelo Jonathan B, Baldosano Tomas V, Maramba-Lazarte Cecilia C
College of Medicine, University of the Philippines Manila.
Institute of Herbal Medicine, National Institutes of Health, University of the Philippines Manila.
Acta Med Philipp. 2024 Oct 15;58(18):91-97. doi: 10.47895/amp.vi0.6160. eCollection 2024.
(Aunasin) is an endemic Philippine plant of the family Primulaceae, with several studies showing the genus Ardisia as having potential antibacterial, antiangiogenic, cytotoxic, and antipyretic properties.
This study aims to determine the antibacterial and antibiofilm-forming activity of ethanolic and aqueous extracts on , Methicillin-Sensitive (MSSA), and Methicillin-Resistant (MRSA).
This is an experimental study testing the activity against bacterial strains of , MSSA, and MRSA using ethanolic and aqueous extracts of leaves. Microtiter susceptibility and biofilm inhibition assays were done with two-fold dilutions of the extract against the selected strains using spectrophotometry with optical density (OD) at 600 nm and 595 nm, respectively, to quantify bacterial growth and biofilm inhibition. The bacterial susceptibility and biofilm inhibition activity was reported as percent inhibition (PI). Minimum inhibitory concentration (MIC), and minimum biofilm inhibition concentration (MBIC) values were obtained using logarithmic regression of the PI values.
ethanolic extracts showed weak growth inhibitory activity against MSSA and MRSA with minimum inhibitory concentration (MIC) values of 2.6192 and 3.2988 mg/mL, respectively, but no biofilm inhibition activity was noted, while the aqueous extracts exhibited negligible biofilm inhibition activity against MSSA and MRSA with minimum biofilm inhibition concentration (MBIC) values of 13.5972 and 8964.82 mg/mL, respectively, and with no growth inhibition activity. Both ethanolic and aqueous extracts showed no growth inhibition and biofilm inhibition activities against .
is susceptible to the bioactivity of the leaf extracts of and has potential to be used as an antibacterial in the treatment of infectious diseases.
（奥纳辛）是菲律宾特有的报春花科植物，多项研究表明紫金牛属具有潜在的抗菌、抗血管生成、细胞毒性和解热特性。
本研究旨在测定乙醇提取物和水提取物对甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌及形成生物膜抑制活性。
这是一项实验研究，使用[植物名称]叶的乙醇提取物和水提取物测试对[细菌名称]、MSSA和MRSA菌株的活性。采用微量滴定药敏试验和生物膜抑制试验，将提取物用两倍稀释法作用于所选菌株，分别使用分光光度法在600nm和595nm处测定光密度（OD），以量化细菌生长和生物膜抑制情况。细菌药敏和生物膜抑制活性以抑制率（PI）表示。通过对PI值进行对数回归获得最低抑菌浓度（MIC）和最低生物膜抑制浓度（MBIC）值。
乙醇提取物对MSSA和MRSA显示出较弱的生长抑制活性，最低抑菌浓度（MIC）值分别为2.6192和3.2988mg/mL，但未观察到生物膜抑制活性；而水提取物对MSSA和MRSA表现出可忽略不计的生物膜抑制活性，最低生物膜抑制浓度（MBIC）值分别为13.5972和8964.82mg/mL，且无生长抑制活性。乙醇提取物和水提取物对[细菌名称]均未显示生长抑制和生物膜抑制活性。
[细菌名称]对[植物名称]叶提取物的生物活性敏感，有潜力用作治疗传染病的抗菌剂。
