State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
Nat Prod Res. 2024 Jan-Feb;38(2):177-185. doi: 10.1080/14786419.2022.2111562. Epub 2022 Aug 12.
Two new natural products named 5,7-dihydroxy-3,3',6,8-tetramethoxy-4',5'-methylenedioxyflavone () and 3,3',5,7-tetramethoxy-4',5'-methylenedioxyflavone (), along with thirteen known compounds, β-sitosterol (), desmethoxyyangonin (), hexadecane (), 3,9-bis(2,4-di-tert-butylphenoxy)-2,4,8,10-tetraoxa-3,9-diphosphaspiro [5.5] undecane 3,9-dioxide (), 2'6'-dihydroxy-4'-methoxydihydrochalcone (), cardamonin (), 3,3',5,6,7,8-hexamethoxy-4',5'-methylenedioxyflavone (), isofraxidin (), aniba dimer A (), 3,3',4',5,5',8-hexamethoxy-6,7-methylenedioxyflavone (), quercetin (), quercitrin () and isoquercitrin () were isolated from (Thunb.) Nakai by various chromatographic methods. Compounds , , , , , and were isolated from for the first time. Their chemical structures were identified through the analysis of NMR and HR-MS spectra. The anti-inflammatory and cytotoxic activities of compounds were evaluated in cell assays. The results indicated that compounds , , , , , and significantly inhibited the NO production in LPS-induced RAW 264.7 murine macrophage cells. Moreover, compounds , , , , , , and exhibited a cytotoxic effect on the human HepG2 cell line.
从（Thunb.）Nakai 中通过各种色谱方法分离得到了两个新的天然产物，分别命名为 5,7-二羟基-3,3',6,8-四甲氧基-4',5'-亚甲二氧基黄酮（）和 3,3',5,7-四甲氧基-4',5'-亚甲二氧基黄酮（），以及 13 个已知化合物，β-谷甾醇（）、去甲氧基姜黄素（）、十六烷（）、3,9-双（2,4-二叔丁基苯氧基）-2,4,8,10-四氧杂-3,9-二磷杂螺 [5.5] 十一烷 3,9-二氧化物（）、2'6'-二羟基-4'-甲氧基二氢查耳酮（）、卡达明（）、3,3',5,6,7,8-六甲氧基-4',5'-亚甲二氧基黄酮（）、异夫拉定（）、阿尼巴二聚体 A（）、3,3',4',5,5',8-六甲氧基-6,7-亚甲二氧基黄酮（）、槲皮素（）、槲皮苷（）和异槲皮苷（）。化合物、、、、和是首次从中分离得到的。通过对 NMR 和 HR-MS 光谱的分析确定了它们的化学结构。在细胞试验中评价了化合物的抗炎和细胞毒性活性。结果表明，化合物、、、、、和显著抑制了 LPS 诱导的 RAW 264.7 鼠巨噬细胞中 NO 的产生。此外，化合物、、、、、、和对人 HepG2 细胞系表现出细胞毒性作用。
