New 4',5'-methylenedioxyflavone derivatives from the whole plant of .

Two new natural products named 5,7-dihydroxy-3,3',6,8-tetramethoxy-4',5'-methylenedioxyflavone () and 3,3',5,7-tetramethoxy-4',5'-methylenedioxyflavone (), along with thirteen known compounds, β-sitosterol (), desmethoxyyangonin (), hexadecane (), 3,9-bis(2,4-di-tert-butylphenoxy)-2,4,8,10-tetraoxa-3,9-diphosphaspiro [5.5] undecane 3,9-dioxide (), 2'6'-dihydroxy-4'-methoxydihydrochalcone (), cardamonin (), 3,3',5,6,7,8-hexamethoxy-4',5'-methylenedioxyflavone (), isofraxidin (), aniba dimer A (), 3,3',4',5,5',8-hexamethoxy-6,7-methylenedioxyflavone (), quercetin (), quercitrin () and isoquercitrin () were isolated from (Thunb.) Nakai by various chromatographic methods. Compounds , , , , , and were isolated from for the first time. Their chemical structures were identified through the analysis of NMR and HR-MS spectra. The anti-inflammatory and cytotoxic activities of compounds were evaluated in cell assays. The results indicated that compounds , , , , , and significantly inhibited the NO production in LPS-induced RAW 264.7 murine macrophage cells. Moreover, compounds , , , , , , and exhibited a cytotoxic effect on the human HepG2 cell line.