Lee Sang-Yeop, Yun Sung Ho, Lee Hayoung, Lee Yun Gyeong, Seo Giwan, Kim Nam Hoon, Park Edmond Changkyun, Lee Chang-Seop, Kim Seung Il
Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Ochang, 28119, Republic of Korea.
Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea.
Clin Proteomics. 2022 Aug 13;19(1):32. doi: 10.1186/s12014-022-09368-8.
Dabie bandavirus, also termed as severe fever with thrombocytopenia syndrome virus (SFTSV), was first isolated in China in 2010. At this time, the virus was found to have spread to South Korea, Japan, and other countries. A high case fatality rate is reported for SFTS, ranging from 12-50% within various sources. Several omics for clinical studies among SFTS patients as well as studies of cultured SFTSV have attempted to characterize the relevant molecular biology and epidemiology of the disease. However, a global serum proteomics analysis among SFTS patients has not yet been reported to date.
In the current study, we evaluated comparative serum proteomics among SFTS patients (eight recovered patients and three deceased patients) with the goal of identifying the protein expression patterns associated with the clinical manifestations of SFTS.
The proteomic results in the current study showed that the coagulation factor proteins, protein S and protein C, were statistically significantly downregulated among the deceased patients. Downregulation of the complement system as well as prolonged neutrophil activation were also observed. Additionally, the downstream proteins of tumour necrosis factor alpha, neutrophil-activating cytokine, and interleukin-1β, an inflammatory cytokine, were overexpressed.
Thrombocytopenia and multiple organ failure are the major immediate causes of death among SFTS patients. In this study, serum proteomic changes related to thrombocytopenia, abnormal immune response, and inflammatory activation were documented in SFTS patients. These findings provide useful information for understanding the clinical manifestations of SFTS.
大别山带病毒，也称为严重发热伴血小板减少综合征病毒（SFTSV），于2010年在中国首次分离出来。当时，发现该病毒已传播到韩国、日本和其他国家。据报道，SFTS的病死率很高，不同来源的病死率在12%至50%之间。针对SFTS患者的多项临床组学研究以及培养的SFTSV研究试图描述该疾病的相关分子生物学和流行病学特征。然而，迄今为止，尚未报道过对SFTS患者进行的全球血清蛋白质组学分析。
在本研究中，我们评估了SFTS患者（8例康复患者和3例死亡患者）之间的血清蛋白质组学比较，目的是确定与SFTS临床表现相关的蛋白质表达模式。
本研究的蛋白质组学结果显示，凝血因子蛋白、蛋白S和蛋白C在死亡患者中统计学上显著下调。还观察到补体系统下调以及中性粒细胞活化延长。此外，肿瘤坏死因子α、中性粒细胞活化细胞因子和炎症细胞因子白细胞介素-1β的下游蛋白过度表达。
血小板减少和多器官功能衰竭是SFTS患者死亡的主要直接原因。在本研究中，记录了SFTS患者中与血小板减少、异常免疫反应和炎症激活相关的血清蛋白质组学变化。这些发现为理解SFTS的临床表现提供了有用信息。
