Zhong Nan-Fang, Huang Hui-Hui, Wei Jiang-Chun, Yang Yong-Cheng, Gao Xiao-Xu, Wei Xiu-Yan, Wang An-Hua, Jia Jing-Ming
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Phytochemistry. 2022 Nov;203:113372. doi: 10.1016/j.phytochem.2022.113372. Epub 2022 Aug 11.
Nine previously undescribed diterpenoids, euphorfiatnoids A-I, together with seven known diterpenoids, were isolated from the roots of wild Euphorbia fischeriana. Their structures were elucidated by the interpretation of HRESIMS, UV, and NMR data. Their configurations were determined by electronic circular dichroism (ECD) spectroscopy analysis and the structure of euphorfiatnoid A was confirmed by X-ray crystallography. To further understand the antitumor effects of E. fischeriana, we tested the cytotoxicity of these compounds against H460, HepG2, and MCF-7 cell lines in vitro using MTT assays. Euphorfiatnoid B exhibited the most promising inhibitory effect against H460 cells with an IC value of 9.97 μM. Euphorfiatnoid A and C also exhibited moderate cytotoxicity against HepG2 cells with IC values of 11.64 and 13.10 μM, respectively.
从野生狼毒大戟的根部分离出9个新的二萜类化合物，即狼毒大戟素A - I，以及7个已知的二萜类化合物。通过高分辨电喷雾电离质谱（HRESIMS）、紫外光谱（UV）和核磁共振波谱（NMR）数据解析阐明了它们的结构。通过电子圆二色光谱（ECD）分析确定了它们的构型，并通过X射线晶体学确定了狼毒大戟素A的结构。为了进一步了解狼毒大戟的抗肿瘤作用，我们使用MTT法在体外测试了这些化合物对H460、HepG2和MCF - 7细胞系的细胞毒性。狼毒大戟素B对H460细胞表现出最显著的抑制作用，IC值为9.97 μM。狼毒大戟素A和C对HepG2细胞也表现出中等细胞毒性，IC值分别为11.64和13.10 μM。
