Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.
Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA; Department of Molecular Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA, USA.
Trends Immunol. 2022 Sep;43(9):741-756. doi: 10.1016/j.it.2022.07.005. Epub 2022 Aug 11.
Next-generation sequencing (NGS) technologies have greatly expanded the size of the known transcriptome. Many newly discovered transcripts are classified as long noncoding RNAs (lncRNAs) which are assumed to affect phenotype through sequence and structure and not via translated protein products despite the vast majority of them harboring short open reading frames (sORFs). Recent advances have demonstrated that the noncoding designation is incorrect in many cases and that sORF-encoded peptides (SEPs) translated from these transcripts are important contributors to diverse biological processes. Interest in SEPs is at an early stage and there is evidence for the existence of thousands of SEPs that are yet unstudied. We hope to pique interest in investigating this unexplored proteome by providing a discussion of SEP characterization generally and describing specific discoveries in innate immunity.
下一代测序 (NGS) 技术极大地扩展了已知转录组的大小。许多新发现的转录本被归类为长非编码 RNA (lncRNA)，尽管它们中的绝大多数都含有短开放阅读框 (sORF)，但人们认为它们通过序列和结构而不是通过翻译的蛋白质产物来影响表型。最近的研究进展表明，在许多情况下，非编码的指定是不正确的，并且这些转录本翻译的 sORF 编码肽 (SEPs) 是多种生物过程的重要贡献者。人们对 SEPs 的兴趣处于早期阶段，并且有证据表明存在数千种尚未研究的 SEPs。我们希望通过一般讨论 SEP 特征并描述先天免疫中的特定发现来激发人们对研究这个未开发的蛋白质组的兴趣。
