Chen Pinpin, Li Shijie, Zhou Zutao, Wang Xu, Shi Deshi, Li Zili, Li Xiaowen, Xiao Yuncai
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Front Microbiol. 2022 Jul 27;13:958112. doi: 10.3389/fmicb.2022.958112. eCollection 2022.
TL (B.A-TL) is well-known for its capability of promoting protein synthesis and lipid metabolism, in particular, the abdominal fat deposition in broilers. However, the underlying molecular mechanism remains unclear. In our study, the regulations of lipid metabolism of broilers by B.A-TL were explored both and . The metabolites of B.A-TL were used to simulate the effect of B.A-TL on liver metabolism based on the chicken hepatocellular carcinoma cell line (i.e., LMH cells). The effects of B.A-TL on lipid metabolism by regulating insulin/IGF signaling pathways were investigated by applying the signal pathway inhibitors . The results showed that the B.A-TL metabolites enhanced hepatic lipid synthesis and stimulated the secretion of IGF-1. The liver transcriptome analysis revealed the significantly upregulated expressions of four genes (, and ) in the B.A-TL treatment group, mainly involved in carbohydrate digestion and absorption as well as biomacromolecule metabolism, with a particularly prominent effect on fatty acid synthase (FASN). Results of cellular assays showed that B.A-TL metabolites were involved in the insulin/IGF signaling pathway, regulating the expressions of lipid metabolism genes (e.g., α, and ) and the FASN protein, ultimately regulating the lipid metabolism the IGF/PI3K/FASN pathway in broilers.
巴豆酰基-肉碱（B.A-TL）以其促进蛋白质合成和脂质代谢的能力而闻名，特别是在肉鸡腹部脂肪沉积方面。然而，其潜在的分子机制仍不清楚。在我们的研究中，从体内和体外两方面探索了B.A-TL对肉鸡脂质代谢的调节作用。基于鸡肝癌细胞系（即LMH细胞），使用B.A-TL的代谢产物来模拟B.A-TL对肝脏代谢的影响。通过应用信号通路抑制剂，研究了B.A-TL通过调节胰岛素/胰岛素样生长因子（IGF）信号通路对脂质代谢的影响。结果表明，B.A-TL的代谢产物增强了肝脏脂质合成并刺激了IGF-1的分泌。肝脏转录组分析显示，在B.A-TL处理组中，四个基因（、和）的表达显著上调，主要参与碳水化合物消化吸收以及生物大分子代谢，对脂肪酸合酶（FASN）的影响尤为突出。细胞试验结果表明，B.A-TL的代谢产物参与胰岛素/IGF信号通路，调节脂质代谢基因（如α、和）以及FASN蛋白的表达，最终通过IGF/PI3K/FASN途径调节肉鸡的脂质代谢。
