Dzhagan Volodymyr, Kapush Olga, Plokhovska Svitlana, Buziashvili Anastasiya, Pirko Yaroslav, Yeshchenko Oleg, Yukhymchuk Volodymyr, Yemets Alla, Zahn Dietrich R T
V. Lashkaryov Institute of Semiconductors Physics, National Academy of Sciences of Ukraine Kyiv Ukraine
Physics Department, Taras Shevchenko National University of Kyiv 01601 Kyiv Ukraine.
RSC Adv. 2022 Aug 3;12(33):21591-21599. doi: 10.1039/d2ra03605c. eCollection 2022 Jul 21.
We report a new pathway for the synthesis of plasmonic gold nanoparticles (Au NPs) in a bio-compatible medium. A modified room temperature approach based on the standard Turkevich synthesis, using sodium citrate as a reducing and stabilizing agent, results in a highly stable colloidal suspension of Au NPs in dimethyl sulfoxide (DMSO). The mean NP size of about 15 nm with a fairly low size distribution is revealed by scanning electron microscopy. The stability test through UV-vis absorption spectroscopy indicates no sign of aggregation for months. The Au NPs are also characterized by X-ray photoelectron, Raman scattering, and FTIR spectroscopies. The stabilisation mechanism of the Au NPs in DMSO is concluded to be similar to that of NPs synthesized in water. The Au NPs obtained in this work are applicable as SERS substrates, as proved by common analytes. In terms of bio-applications, they do not possess such side-effects as pronounced antibacterial activity, based on the tests performed on non-pathogenic Gram-positive or Gram-negative bacteria.
我们报道了一种在生物相容性介质中合成等离子体金纳米颗粒（Au NPs）的新途径。基于标准的Turkevich合成法，采用柠檬酸钠作为还原剂和稳定剂，改进的室温方法可在二甲基亚砜（DMSO）中得到高度稳定的Au NPs胶体悬浮液。扫描电子显微镜显示平均NP尺寸约为15 nm，尺寸分布相当低。通过紫外可见吸收光谱进行的稳定性测试表明，数月内没有聚集迹象。Au NPs还通过X射线光电子能谱、拉曼散射光谱和傅里叶变换红外光谱进行了表征。得出结论，Au NPs在DMSO中的稳定机制与在水中合成的NPs相似。如常见分析物所证明的，本工作中获得的Au NPs可用作表面增强拉曼散射（SERS）底物。就生物应用而言，基于对非致病性革兰氏阳性或革兰氏阴性细菌进行的测试，它们不具有明显的抗菌活性等副作用。
