Wang Chao, Chi Hui, Zhang Fan, Wang Xinyue, Wang Jiarui, Zhang Hao, Liu Ying, Huang Xiaona, Bai Yungang, Xu Kun, Wang Pixin
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 PR China
University of Science and Technology of China Hefei 230026 PR China.
Chem Sci. 2022 Jun 10;13(30):8766-8772. doi: 10.1039/d2sc01746f. eCollection 2022 Aug 4.
The field of biocatalysis is expanding owing to the increasing demand for efficient low-cost green chemical processes. However, a feasible strategy for achieving product separation, enzyme recovery, and high catalytic efficiency in biocatalysis remains elusive. Herein, we present thermoresponsive Pickering high internal phase emulsions (HIPEs) as controllable scaffolds for efficient biocatalysis; these HIPEs demonstrate a transition between emulsification and demulsification depending on temperature. Ultra-high-surface-area Pickering HIPEs were stabilized by Candida antarctica lipase B immobilized on starch particles modified with butyl glycidyl ether and glycidyl trimethyl ammonium chloride, thus simplifying the separation and reuse processes and significantly improving the catalytic efficiency. In addition, the switching temperature can be precisely tuned by adjusting the degree of substitution of the modified starches to meet the temperature demands of various enzymes. We believe that this system provides a green platform for various interfacial biocatalytic processes of industrial interest.
由于对高效低成本绿色化学工艺的需求不断增加，生物催化领域正在不断扩展。然而，在生物催化中实现产物分离、酶回收和高催化效率的可行策略仍然难以捉摸。在此，我们提出了热响应性Pickering高内相乳液（HIPE）作为高效生物催化的可控支架；这些HIPE根据温度表现出乳化和破乳之间的转变。通过固定在经丁基缩水甘油醚和缩水甘油三甲基氯化铵改性的淀粉颗粒上的南极假丝酵母脂肪酶B来稳定超高表面积Pickering HIPE，从而简化了分离和再利用过程，并显著提高了催化效率。此外，通过调节改性淀粉的取代度可以精确调节转变温度，以满足各种酶的温度需求。我们相信，该系统为各种具有工业价值的界面生物催化过程提供了一个绿色平台。
