Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, China.
The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China.
J Affect Disord. 2022 Nov 15;317:166-175. doi: 10.1016/j.jad.2022.08.027. Epub 2022 Aug 18.
This study aimed to explore the gender specificity of gut microbiome in patients with unipolar and bipolar depression disorder by analyzing the data of gut microbiome in this two mental disorders and healthy people.
A case-control study using 16S ribosomal RNA gene sequencing from fecal samples of MDD (male set, n = 43; female set, n = 77) and BD (male set, n = 82; female set, n = 83) compared with HCs (male set, n = 71; female set, n = 100) was conducted. Linear discriminant analysis was used to identify microbial characteristics. Through cooccurrence analysis, the potential correlations of the differential gut microbiota in different genders was explored. Finally, the gender-specific distinguishing microorganisms were identified as biomaker, and the diagnostic performance was verified by five-fold cross validation.
A specific cluster was found enriched only in female MDD set, including 4 Bacteroideae OTUs. Similarly, 3 Lachnospiraceae OTUs was found significantly increased in female BD compared with other groups. In addition, the consistent enrichment of Pseudomonadacea in male and female may be the characteristic disease-related gut microbiota of BD. Besides, the diagnostic potential of gender specific biomarker panel in male (male validation AUC: 0.758-0.874, accurancy: 0.693-0.792; female validation AUC: 0.727-0.883, accurancy: 0.678-0.781) and female (male validation AUC: 0.787-0.883, accurancy: 0.719-0.784; female validation AUC: 0.795-0.898, accurancy: 0.689-0.838) has also been identified and confirmed.
The microbiological changes in both MDD and BD are sex specific, and gender specific biomarker panel has better diagnostic performance, which provide a certain reference in sex difference for future clinical differentiation and microbial intervention.
通过分析单相和双相抑郁症患者的肠道微生物组数据，探讨肠道微生物组在单相和双相抑郁症患者中的性别特异性。
采用 16S 核糖体 RNA 基因测序，对 MDD（男性组，n=43；女性组，n=77）和 BD（男性组，n=82；女性组，n=83）患者与健康对照者（男性组，n=71；女性组，n=100）的粪便样本进行病例对照研究。采用线性判别分析识别微生物特征。通过共生分析，探讨不同性别差异肠道微生物之间的潜在相关性。最后，将性别特异性鉴别微生物鉴定为生物标志物，并通过五重交叉验证验证其诊断性能。
仅在女性 MDD 组中发现了一个特定的聚类，包括 4 个拟杆菌门 OTUs。同样，与其他组相比，女性 BD 中发现 3 个lachnospiraceae OTUs 显著增加。此外，假单胞菌属在男性和女性中的一致富集可能是 BD 与疾病相关的肠道微生物群的特征。此外，男性（男性验证 AUC：0.758-0.874，准确性：0.693-0.792；女性验证 AUC：0.727-0.883，准确性：0.678-0.781）和女性（男性验证 AUC：0.787-0.883，准确性：0.719-0.784；女性验证 AUC：0.795-0.898，准确性：0.689-0.838）的性别特异性生物标志物谱也得到了识别和验证。
MDD 和 BD 的微生物变化均具有性别特异性，且性别特异性生物标志物谱具有更好的诊断性能，为今后临床鉴别和微生物干预提供了一定的性别差异参考。
