壳聚糖负载 - 香豆酸纳米粒子的抗氧化、抗炎、抗菌和抗糖尿病活性。
Antioxidant, Anti-inflammatory, Antimicrobial, and Antidiabetic Activities of Synthesized Chitosan-loaded -Coumaric Acid Nanoparticles.
机构信息
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, 608002, Tamil Nadu, India.
Department of Chemistry, Faculty of Science, Annamalai University, Annamalainagar, 608002, Tamil Nadu, India.
出版信息
Curr Pharm Biotechnol. 2023;24(9):1178-1194. doi: 10.2174/1389201023666220822112923.
BACKGROUND
-Coumaric acid is a phenolic compound widely distributed in fruits and vegetables that displays an array of therapeutic properties, including antidiabetic effects. Prominent application in diabetes is limited due to its suboptimal pharmacokinetics, poor aqueous solubility, and poor bioavailability. Nanotechnology-based delivery methods have been developed to address these limitations and improve the therapeutic uses of -coumaric acid, and the nanoencapsulation method is emerging as a feasible alternative.
OBJECTIVE
The objective of this study is to synthesize -coumaric acid nanoparticles (PCNPs) and to evaluate their activities.
METHODS
The PCNPs were synthesized by the nanoprecipitation method and characterized by UV-visible spectroscopy, zeta potential, Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) with dispersive energy X-ray (EDX) analysis. In addition, the PCNPs were analyzed for antioxidant activity using six different free radical scavenging assays and were also analyzed for antimicrobial, anti-inflammatory, antithrombotic, and antidiabetic effects.
RESULTS
The formation of PCNPs was confirmed by UV-visible spectra at 283 nm, and FTIR analysis revealed the reduction and capping of the chitosan nanoparticles. SEM was used to assess the size and shape of the PCNPs, and the high absorption property of the PCNPs was investigated using EDX analysis. The PCNPs had significant antioxidant, hydrogen peroxide (HO), lipid peroxidation (LPO), superoxide and nitric oxide (NO) radical scavenging power activities, and showed potent antimicrobial, anti-inflammatory, antithrombotic, and antidiabetic activities.
CONCLUSION
The present study suggests that PCNPs can be used as a potential medication delivery approach to provide a greater nephroprotective effect in the treatment of diabetic nephropathy. To the best of our knowledge, this is the first attempt at the synthesis of chitosan-loaded PCNPs.
背景
-香豆酸是一种广泛分布于水果和蔬菜中的酚类化合物，具有多种治疗特性，包括抗糖尿病作用。由于其药代动力学不佳、水溶性差和生物利用度低，其在糖尿病中的突出应用受到限制。基于纳米技术的递药方法已经被开发出来，以解决这些限制并提高 -香豆酸的治疗用途，纳米囊化方法正在成为一种可行的替代方法。
目的
本研究旨在合成 -香豆酸纳米颗粒（PCNPs）并评估其活性。
方法
通过纳米沉淀法合成 PCNPs，并通过紫外-可见光谱、Zeta 电位、傅里叶变换红外（FTIR）光谱和扫描电子显微镜（SEM）以及分散能量 X 射线（EDX）分析进行表征。此外，还通过六种不同的自由基清除测定法分析了 PCNPs 的抗氧化活性，并分析了其抗菌、抗炎、抗血栓和抗糖尿病作用。
结果
通过 283nm 的紫外-可见光谱证实了 PCNPs 的形成，FTIR 分析显示壳聚糖纳米颗粒的还原和封端。使用 SEM 评估 PCNPs 的大小和形状，并通过 EDX 分析研究 PCNPs 的高吸收特性。PCNPs 具有显著的抗氧化、过氧化氢（HO）、脂质过氧化（LPO）、超氧化物和一氧化氮（NO）自由基清除能力，并表现出有效的抗菌、抗炎、抗血栓和抗糖尿病作用。
结论
本研究表明，PCNPs 可用作潜在的药物传递方法，在治疗糖尿病肾病方面提供更大的肾脏保护作用。据我们所知，这是首次尝试合成壳聚糖负载的 PCNPs。
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