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鸡α-D-珠蛋白基因5'端DNase I高敏区域内的蛋白质结合位点。

Protein-binding sites within the 5' DNase I-hypersensitive region of the chicken alpha D-globin gene.

作者信息

Kemper B, Jackson P D, Felsenfeld G

出版信息

Mol Cell Biol. 1987 Jun;7(6):2059-69. doi: 10.1128/mcb.7.6.2059-2069.1987.

DOI:10.1128/mcb.7.6.2059-2069.1987
PMID:3600658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365326/
Abstract

We mapped at high resolution and as a function of development the hypersensitive domain in the 5'-flanking region of the chicken alpha D-globin gene and determined the specific protein-binding sites within the domain. The domain extends from -130 to +80 nucleotides (nt) relative to the cap site. DNase I footprinting within intact embryonic erythrocyte nuclei revealed a strongly protected area from -71 to -52 nt. The same area was weakly protected in adult nuclei. A factor was present in extracts of erythrocyte nuclei from both embryos and adults that protected the sequence AAGATAAGG (-63 to -55 nt) in DNase I footprinting experiments; at higher concentrations of extract, sequences immediately adjacent (-73 to -64 and -53 to -38) were also protected. The same pattern of binding was revealed by gel mobility shift assays. The identical AAGATAAGG sequence is found in the 5'-flanking region of the beta rho gene; it competed for binding of the alpha D-specific factor, suggesting that regulatory elements are shared.

摘要

我们以高分辨率绘制了鸡α D-珠蛋白基因5'-侧翼区域超敏结构域随发育的变化图谱，并确定了该结构域内的特异性蛋白质结合位点。该结构域相对于帽位点从-130至+80个核苷酸（nt）延伸。完整胚胎红细胞核内的DNase I足迹分析显示，-71至-52 nt区域受到强烈保护。在成年细胞核中，同一区域受到的保护较弱。胚胎和成年红细胞核提取物中均存在一种因子，在DNase I足迹分析实验中，该因子保护序列AAGATAAGG（-63至-55 nt）；在提取物浓度较高时，紧邻的序列（-73至-64和-53至-38）也受到保护。凝胶迁移率变动分析揭示了相同的结合模式。在β rho基因的5'-侧翼区域发现了相同的AAGATAAGG序列；它竞争α D特异性因子的结合，表明调控元件是共享的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/267b5c49df07/molcellb00078-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/e8a9eb6baaaa/molcellb00078-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/35d7e111d1be/molcellb00078-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/6da3ddd9cc8e/molcellb00078-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/e86da67df7e0/molcellb00078-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/6b66d82730be/molcellb00078-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/3ae9474759bc/molcellb00078-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/267b5c49df07/molcellb00078-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/e8a9eb6baaaa/molcellb00078-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/35d7e111d1be/molcellb00078-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/6da3ddd9cc8e/molcellb00078-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/e86da67df7e0/molcellb00078-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/6b66d82730be/molcellb00078-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/3ae9474759bc/molcellb00078-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/365326/267b5c49df07/molcellb00078-0039-a.jpg

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