Evans T, Reitman M, Felsenfeld G
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80. doi: 10.1073/pnas.85.16.5976.
We have identified a protein present only in erythroid cells that binds to two adjacent sites within an enhancer region of the chicken beta-globin locus. Mutation of the sites, so that binding by the factor can no longer be detected in vitro, leads to a loss of enhancing ability, assayed by transient expression in primary erythrocytes. Binding sites for the erythroid-specific factor (Eryf1) are found within regulatory regions for all chicken globin genes. A strong Eryf1 binding site is also present within the enhancer of at least one human globin gene, and proteins from human erythroid cells (but not HeLa cells) bind to both the chicken and the human sites.
我们已经鉴定出一种仅存在于红细胞中的蛋白质，它能与鸡β-珠蛋白基因座增强子区域内的两个相邻位点结合。这些位点发生突变后，在体外无法再检测到该因子的结合，通过原代红细胞中的瞬时表达检测，这会导致增强能力丧失。在所有鸡珠蛋白基因的调控区域内都发现了红细胞特异性因子（Eryf1）的结合位点。在至少一个人类珠蛋白基因的增强子内也存在一个强Eryf1结合位点，并且来自人类红细胞（而非HeLa细胞）的蛋白质能与鸡和人类的位点结合。
