Mura Mathilde, Weiss-Gayet Michèle, Della-Schiava Nellie, Chirico Erica, Lermusiaux Patrick, Chambion-Diaz Marie, Faes Camille, Boreau Anaelle, Chazaud Bénédicte, Millon Antoine, Pialoux Vincent
Interuniversity Laboratory of Human Movement Biology EA7424, Université Claude Bernard Lyon 1, Université de Lyon, 69008 Lyon, France.
Institut NeuroMyoGene, CNRS UMR 5310, INSERM U1217, Université Claude Bernard Lyon 1, Université de Lyon, 69008 Lyon, France.
Antioxidants (Basel). 2022 Aug 5;11(8):1529. doi: 10.3390/antiox11081529.
Atherosclerosis is associated with low-grade inflammation involving circulating monocytes. It has been shown that the levels of intermediate pro-inflammatory monocytes are associated with cardiovascular mortality and risk of ischemic stroke. It also has been shown that physical activity (PA) decreases inflammation markers, incidence of strokes, and mortality. In this cross-sectional study, we tested the effect of PA on circulating monocytes phenotype rate. A total of 29 patients with a carotid stenosis > 50% were recruited. Levels of physical activity (MET.min/week) were measured by the GPAQ questionnaire, arterial samples of blood were collected to analyze monocyte phenotype (classical, intermediate and non-classical) assessed by flow cytometry, and venous blood samples were used to dose antioxidant activity and oxidative damage. Antioxidant capacity was reduced and oxidative damage increased in patients. There was a significant decrease in the percentage of classical and intermediate monocytes in moderately active patients as compared with non-active and highly active patients. Inversely, the rate of non-classical monocytes increased in moderately active patients. Intense PA appears to blunt the beneficial effects of moderate PA. Our study also suggests that PA could be beneficial in such patients by reducing the rate of intermediate monocytes known to predict the risk of ischemic stroke and by increasing the non-classical monocytes involved in lesions’ healing. Nevertheless, a longitudinal study would be necessary to confirm this hypothesis.
动脉粥样硬化与涉及循环单核细胞的低度炎症相关。研究表明，中间促炎单核细胞的水平与心血管死亡率和缺血性中风风险相关。研究还表明，体育活动（PA）可降低炎症标志物、中风发病率和死亡率。在这项横断面研究中，我们测试了PA对循环单核细胞表型率的影响。共招募了29例颈动脉狭窄>50%的患者。通过GPAQ问卷测量体育活动水平（MET·分钟/周），采集动脉血样本以分析通过流式细胞术评估的单核细胞表型（经典型、中间型和非经典型），并使用静脉血样本测定抗氧化活性和氧化损伤。患者的抗氧化能力降低，氧化损伤增加。与不活动和高度活动的患者相比，中度活动患者的经典单核细胞和中间单核细胞百分比显著降低。相反，中度活动患者的非经典单核细胞率增加。剧烈PA似乎会削弱中度PA的有益效果。我们的研究还表明，PA可能对这类患者有益，通过降低已知可预测缺血性中风风险的中间单核细胞率，并增加参与损伤愈合的非经典单核细胞。然而，需要进行纵向研究来证实这一假设。
