Dufour Cécile, Villa-Rodriguez Jose A, Furger Christophe, Lessard-Lord Jacob, Gironde Camille, Rigal Mylène, Badr Ashraf, Desjardins Yves, Guyonnet Denis
Anti Oxidant Power AOP/MH2F-LAAS/CNRS, 7 Avenue du Colonel Roche, BP 54200, 31031 Toulouse, France.
Business Incubation Group, Symrise Taste, Nutrition & Health, 1E Allée Ermengarde d'Anjou, 35000 Rennes, France.
Antioxidants (Basel). 2022 Aug 12;11(8):1561. doi: 10.3390/antiox11081561.
Oxidative stress and chronic inflammation contribute to some chronic diseases. Aronia berries are rich in polyphenols. The aim of the present study was to characterize the cellular antioxidant effect of an aronia extract to reflect the potential physiological in vivo effect. Cellular in vitro assays in three cell lines (Caco-2, HepG2, and SH-SY5Y) were used to measure the antioxidant effect of AE, in three enriched polyphenolic fractions (A1: anthocyanins and phenolic acids; A2: oligomeric proanthocyanidins; A3: polymeric proanthocyanidins), pure polyphenols and microbial metabolites. Both direct (intracellular and membrane radical scavenging, catalase-like effect) and indirect (NRF2/ARE) antioxidant effects were assessed. AE exerted an intracellular free radical scavenging activity in the three cell lines, and A2 and A3 fractions showed a higher effect in HepG2 and Caco-2 cells. AE also exhibited a catalase-like activity, with the A3 fraction having a significant higher activity. Only A1 fraction activated the NRF2/ARE pathway. Quercetin and caffeic acid are the most potent antioxidant polyphenols, whereas cyanidin and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone showed the highest antioxidant effect among polyphenol metabolites. AE rich in polyphenols possesses broad cellular antioxidant effects, and proanthocyanidins are major contributors. Polyphenol metabolites may contribute to the overall antioxidant effect of such extract in vivo.
氧化应激和慢性炎症会引发一些慢性疾病。刺玫果富含多酚。本研究的目的是表征刺玫果提取物的细胞抗氧化作用，以反映其潜在的体内生理效应。利用三种细胞系（Caco-2、HepG2和SH-SY5Y）进行细胞体外试验，以测量刺玫果提取物（AE）、三种富集多酚组分（A1：花青素和酚酸；A2：低聚原花青素；A3：聚合原花青素）、纯多酚和微生物代谢产物的抗氧化作用。评估了直接（细胞内和膜自由基清除、过氧化氢酶样作用）和间接（NRF2/ARE）抗氧化作用。AE在三种细胞系中均表现出细胞内自由基清除活性，且A2和A3组分在HepG2和Caco-2细胞中表现出更高的作用。AE还表现出过氧化氢酶样活性，其中A3组分的活性显著更高。只有A1组分激活了NRF2/ARE通路。槲皮素和咖啡酸是最有效的抗氧化多酚，而花青素和5-(3',4'-二羟基苯基)-γ-戊内酯在多酚代谢产物中表现出最高的抗氧化作用。富含多酚的AE具有广泛的细胞抗氧化作用，原花青素是主要贡献者。多酚代谢产物可能有助于该提取物在体内的整体抗氧化作用。
