Siddik Muhammad A B, Vatsos Ioannis N, Rahman Md Arifur, Pham Hung Duc
Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali 8602, Bangladesh.
Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
Antioxidants (Basel). 2022 Aug 14;11(8):1572. doi: 10.3390/antiox11081572.
The present study examined the efficacy of dietary selenium-enriched spirulina (SeE-SP) on growth performance, antioxidant response, liver and intestinal health, immunity and disease resistance of Asian seabass, Lates calcarifer. A total of 480 seabass juveniles with an initial weight of 9.22 ± 0.09 g/fish were randomly assigned to four dietary groups. The fish were fed a fishmeal protein replacement diets with SeE-SP at 5%, 10%, and 20%, namely SeE-SP5, SeE-SP10, and SeE-SP20, and a fishmeal-based diet as control for 8 weeks. The results indicated that seabass juveniles fed SeE-SP5 and SeE-SP10 diets grew at the same rate as the fish fed a fishmeal-based control diet after 8 weeks of feeding, while SeE-SP20 grew at a significantly lower rate than the control (p < 0.05). Although most of the measured biochemical parameters were not influenced by the Se-SP diets, serum antioxidant-enzyme glutathione peroxidase (GPx) and immunological indices, such as lysozyme activity and immunoglobulin-M, were found significantly higher in the SeE-SP5 and SeE-SP10 diets compared to control. In addition, the fish fed the SeE-SP5 diet showed significantly lower mortalities after the 14-day of bacterial challenge with V. harveyi. These outcomes indicated that up to 10% inclusion of SeE-SP in the diet of juvenile Asian seabass does not compromise growth, while SeE-SP5 enhanced disease resistance in juvenile seabass.
本研究考察了日粮中添加富硒螺旋藻（SeE-SP）对尖吻鲈生长性能、抗氧化反应、肝脏和肠道健康、免疫力及抗病力的影响。选取480尾初始体重为9.22±0.09克/尾的尖吻鲈幼鱼，随机分为四组。分别投喂以SeE-SP替代鱼粉蛋白的日粮，添加量分别为5%、10%和20%，即SeE-SP5、SeE-SP10和SeE-SP20，以鱼粉日粮作为对照，为期8周。结果表明，投喂8周后，投喂SeE-SP5和SeE-SP10日粮的尖吻鲈幼鱼生长速度与投喂鱼粉对照日粮的鱼相同，而SeE-SP20组生长速度显著低于对照组（p<0.05）。虽然大多数测定的生化参数不受Se-SP日粮影响，但与对照组相比，SeE-SP5和SeE-SP10日粮组血清抗氧化酶谷胱甘肽过氧化物酶（GPx）以及免疫指标如溶菌酶活性和免疫球蛋白M显著更高。此外，用哈维氏弧菌进行14天细菌攻毒后，投喂SeE-SP5日粮的鱼死亡率显著更低。这些结果表明，在尖吻鲈幼鱼日粮中添加高达10%的SeE-SP不会影响生长，而SeE-SP5增强了幼鱼的抗病力。
