Kazerooni Elham Ahmed, Maharachchikumbura Sajeewa S N, Al-Sadi Abdullah Mohammed, Rashid Umer, Kang Sang-Mo, Lee In-Jung
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 611731, China.
J Fungi (Basel). 2022 Jul 27;8(8):785. doi: 10.3390/jof8080785.
Fungal strains isolated from the rhizosphere of healthy were examined to mitigate symptoms of drought and salinity stresses. The fungal strains were identified as and based on their DNA sequencing and morphological analysis. Additionally, the fungal strains were assayed for a number of plant growth promoting traits and abiotic stresses on solid media. Moreover, a greenhouse experiment was conducted and tomato seedlings were treated with 25% PEG or 1.5% NaCl for 12 days, and the impact of plant growth promoting fungi (PGPF) on tomato seedling performance under these conditions was examined. PGPF application raised the survival of the stressed tomato plants, which was evidenced by higher physiological and biochemical processes. The PGPF-inoculated plants exhibited higher chlorophyll, carotenoid, protein, amino acid, antioxidant activities, salicylic acid, glucose, fructose, and sucrose contents, and showed lower hydrogen peroxide, and lipid metabolism relative to control plants under stress. Analysis using gene expression showed enhanced expression of gene and reduced expression of , , , and genes following PGPFs application. Overall, the outcomes of this study elucidate the function of these fungal strains and present candidates with potential implementation as biofertilizers and in promoting plant stress endurance.
对从健康植株根际分离出的真菌菌株进行了检测，以减轻干旱和盐胁迫症状。根据DNA测序和形态学分析，这些真菌菌株被鉴定为[具体菌株名称未给出]。此外，在固体培养基上对这些真菌菌株的多种促进植物生长特性和非生物胁迫进行了测定。此外，进行了一项温室试验，用25%聚乙二醇（PEG）或1.5%氯化钠处理番茄幼苗12天，并研究了促植物生长真菌（PGPF）在这些条件下对番茄幼苗性能的影响。PGPF的施用提高了受胁迫番茄植株的存活率，这通过更高的生理和生化过程得到证明。与胁迫下的对照植株相比，接种PGPF的植株表现出更高的叶绿素、类胡萝卜素、蛋白质、氨基酸、抗氧化活性、水杨酸、葡萄糖、果糖和蔗糖含量，并且过氧化氢和脂质代谢较低。基因表达分析表明，施用PGPF后，[具体基因名称未给出]基因表达增强，而[其他具体基因名称未给出]基因表达降低。总体而言，本研究结果阐明了这些真菌菌株的功能，并提出了具有作为生物肥料和促进植物抗逆性潜在应用价值的候选菌株。
