Milic Nikola, Christinaki Anastasia C, Benaki Dimitra, Stavrou Aimilia A, Tsafantakis Nikolaos, Fokialakis Nikolas, Kouvelis Vassili N, Gonou-Zagou Zacharoula
Section of Ecology and Systematics, Department of Biology, National and Kapodistrian University of Athens, Panepistimiopolis, 157 84 Athens, Greece.
Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 157 71 Athens, Greece.
J Fungi (Basel). 2022 Aug 20;8(8):877. doi: 10.3390/jof8080877.
(1) Background: Species of the anamorphic genus , are known for their fungicolous lifestyle, making them important mycopathogens in fungiculture. Many morphological, ecological, and molecular phylogenetic studies of the genus have been done to date, but taxonomic uncertainties and challenges still remain. Fungal secondary metabolites, being vastly diverse, are utilised as an extra tool in fungal systematics. Despite being studied for their potentially bioactive compounds, species are insufficiently investigated regarding metabolomics. (2) Methods: The aim of this study is the identification of Greek strains of by integrating morphological data, ITS-based phylogeny, and H NMR-based metabolomics into a polyphasic approach. (3) Results: Twenty-three strains, isolated from sporophores of macromycetes inhabiting diverse Greek ecosystems, were morphologically identified as , , , , , and (anamorph ), whereas seven strains, which produced red-pigmented metabolites, presented an ambiguous taxonomic position at the species level. Molecular phylogenetics and metabolomics corroborated the morphological findings. (4) Conclusions: Thorough morphological study, ITS region-based phylogeny, and NMR-based metabolomics contribute complementarily to resolving the genus systematics.
(1) 背景：无性型属的物种以其寄生于真菌的生活方式而闻名，这使它们成为真菌养殖中的重要真菌病原体。迄今为止，已经对该属进行了许多形态学、生态学和分子系统发育研究，但分类学上的不确定性和挑战仍然存在。真菌次生代谢产物种类繁多，被用作真菌系统学中的一种额外工具。尽管对其潜在的生物活性化合物进行了研究，但关于该属物种的代谢组学研究仍不充分。(2) 方法：本研究的目的是通过将形态学数据、基于ITS的系统发育和基于1H NMR的代谢组学整合到多相方法中，鉴定希腊的该属菌株。(3) 结果：从栖息于希腊不同生态系统的大型真菌子实体中分离出的23株菌株，在形态上被鉴定为、、、、、和（无性型），而7株产生红色色素代谢产物的菌株在物种水平上呈现出模糊的分类地位。分子系统发育学和代谢组学证实了形态学研究结果。(4) 结论：全面的形态学研究、基于ITS区域的系统发育和基于NMR的代谢组学对解决该属的系统学问题具有互补作用。
