Zoclanclounon Yedomon Ange Bovys, Rostás Michael, Chung Nam-Jin, Mo Youngjun, Karlovsky Petr, Dossa Komivi
Genomics Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea.
Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.
Life (Basel). 2022 Aug 8;12(8):1200. doi: 10.3390/life12081200.
Peroxidases and laccases are oxidative enzymes involved in physiological processes in plants, covering responses to biotic and abiotic stress as well as biosynthesis of health-promoting specialized metabolites. Although they are thought to be involved in the biosynthesis of (+)-pinoresinol, a comprehensive investigation of this class of enzymes has not yet been conducted in the emerging oil crop sesame and no information is available regarding the potential (+)-pinoresinol synthase genes in this crop. In the present study, we conducted a pan-genome-wide identification of peroxidase and laccase genes coupled with transcriptome profiling of diverse sesame varieties. A total of 83 and 48 genes have been identified as coding for sesame peroxidase and laccase genes, respectively. Based on their protein domain and genes used as baits, the genes were classified into nine and seven groups of peroxidase and laccase genes, respectively. The expression of the genes was evaluated using dynamic transcriptome sequencing data from six sesame varieties, including one elite cultivar, white vs black seed varieties, and high vs low oil content varieties. Two peroxidase genes ( and ) and two laccase genes ( and ), well conserved within the sesame pan-genome and exhibiting consistent expression patterns within sesame varieties matching the kinetic of (+)-pinoresinol accumulation in seeds, were identified as potential (+)-pinoresinol synthase genes. Cis-acting elements of the candidate genes revealed their potential involvement in development, hormonal signaling, and response to light and other abiotic triggers. Transcription factor enrichment analysis of promoter regions showed the predominance of binding sequences. The findings from this study pave the way for lignans-oriented engineering of sesame with wide potential applications in food, health and medicinal domains.
过氧化物酶和漆酶是参与植物生理过程的氧化酶，涵盖对生物和非生物胁迫的响应以及促进健康的特殊代谢产物的生物合成。尽管它们被认为参与了（+）-松脂醇的生物合成，但尚未在新兴油料作物芝麻中对这类酶进行全面研究，关于该作物中潜在的（+）-松脂醇合酶基因也没有相关信息。在本研究中，我们对过氧化物酶和漆酶基因进行了泛基因组范围的鉴定，并对不同芝麻品种进行了转录组分析。总共分别鉴定出83个和48个编码芝麻过氧化物酶和漆酶的基因。根据它们的蛋白质结构域和用作诱饵的基因，这些基因分别被分为过氧化物酶基因的九组和漆酶基因的七组。利用来自六个芝麻品种的动态转录组测序数据评估了这些基因的表达，这些品种包括一个优良品种、白籽与黑籽品种以及高油与低油含量品种。两个过氧化物酶基因（和）以及两个漆酶基因（和），在芝麻泛基因组中高度保守，并且在芝麻品种中表现出与种子中（+）-松脂醇积累动力学相匹配的一致表达模式，被鉴定为潜在的（+）-松脂醇合酶基因。候选基因的顺式作用元件揭示了它们可能参与发育、激素信号传导以及对光和其他非生物触发因素的响应。启动子区域的转录因子富集分析显示结合序列占主导。本研究的结果为芝麻以木脂素为导向的工程改造铺平了道路，在食品、健康和医药领域具有广泛的潜在应用。
