Asghar Farah, Bano Abida, Waheed Fadia, Ahmed Anjum Aftab, Ejaz Hasan, Javed Numan
Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan.
Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Saudi J Biol Sci. 2023 Apr;30(4):103613. doi: 10.1016/j.sjbs.2023.103613. Epub 2023 Mar 1.
The frequency of Staphylococcus aureus strains associated with oral cavity microbiota has prodigious consideration. Although S. aureus has been reflected as an ephemeral member of the human oral cavity microbiota, the isolation, identification, and characterization of is important. The present study aimed to characterize S. aureus strains from the oral cavity microflora, isolation of from the human oral cavity microbiota, and demographic information of the participants to evaluate exogenous factors associated with the presence of and their genetic analysis linkage with different factors. The method used in this study is the isolation of oral cavity microbiomes using sheep blood agar and Mannitol salt agar. We performed antibiotic profiling with various antibiotics and genetic analysis utilizing gene-specific primers for specific genes, including , , , , and . A significant number of isolates were found in the oral cavity of humans 18/84 (21.42%), and all 18 strains tested positive for the confirmatory gene. Antibiotic resistance-conferring gene was positive in 10 (55.6%) isolates. It was found that the occurrence of and coagulase ( genes was 9 (50%), 6 (33.33%), and 10 (55.6%), respectively. The genetic analysis reported that significant associations were present between male and gene ( = 0.03) and ( = 0.03), smokers with the occurrence of ( = 0.02), ( = 0.048) and ( = 0.02) genes. Likewise, the association of antibiotic usage was significantly found with ( = 0.02), ( = 0.02); however, the individuals who have taken orthodontic treatment recently have a significant association with ( = 0.017). The use of mouth rinse was significantly associated with the prevalence of the gene ( = 0.01), and tooth brushing frequency and inflammation of the buccal cavity were also statistically significant in relation to gene prevalence ( = 0.02, 0.00, respectively). Moreover, calories and weight-controlled diet were significantly associated with and highly significant with ( = 0.02, 0.048, 0.000), so all < 0.05, and no significant association was found between the socioeconomic status of individuals with aforementioned analyzed genes.
与口腔微生物群相关的金黄色葡萄球菌菌株的频率受到了极大关注。尽管金黄色葡萄球菌已被视为人类口腔微生物群的短暂成员，但对其进行分离、鉴定和表征很重要。本研究旨在表征来自口腔微生物群落的金黄色葡萄球菌菌株，从人类口腔微生物群中分离该菌株，并了解参与者的人口统计学信息，以评估与该菌株存在相关的外部因素及其与不同因素的遗传分析关联。本研究使用的方法是使用羊血琼脂和甘露醇盐琼脂分离口腔微生物群落。我们用各种抗生素进行了抗生素敏感性分析，并利用针对特定基因（包括、、、和）的基因特异性引物进行了遗传分析。在18/84（21.42%）的人类口腔中发现了大量金黄色葡萄球菌分离株，所有18株菌株的确认基因检测均呈阳性。赋予抗生素抗性的基因在10株（55.6%）分离株中呈阳性。发现金黄色葡萄球菌和凝固酶（基因）的出现率分别为9株（50%）、6株（33.33%）和10株（55.6%）。遗传分析报告称，男性与基因（=0.03）和（=0.03）之间、吸烟者与基因（=0.02）、（=0.048）和（=0.02）的出现之间存在显著关联。同样，抗生素使用与（=0.02）、（=0.02）有显著关联；然而，最近接受正畸治疗的个体与（=0.017）有显著关联。使用漱口水与基因的流行率显著相关（=0.01），刷牙频率和颊腔炎症与基因流行率也具有统计学意义（分别为=0.02、0.00）。此外，热量和体重控制饮食与显著相关，与高度显著相关（=0.02、0.048、0.000），所以所有<0.05，并且在个体的社会经济地位与上述分析基因之间未发现显著关联。
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