在体外将重组DNA分子包装到噬菌体颗粒中。
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
作者信息
Hohn B, Murray K
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3259-63. doi: 10.1073/pnas.74.8.3259.
Abstract
Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.
摘要
在与纯化酶的反应中制备的重组噬菌体基因组可通过体外包装到噬菌体头部直接回收。该过程高效且无选择性，并在处理重组DNA的初始阶段提供了限制措施。连接酶[聚（脱氧核糖核苷酸）：聚（脱氧核糖核苷酸）连接酶（形成AMP），EC 6.5.1.1]反应产物在包装过程中可与内源性噬菌体DNA重组，但紫外线照射可消除内源性DNA的生物活性。
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