Jaisankar Abinaya, Krishnan Sasirekha, Rangasamy Loganathan
Drug Discovery Unit, Centre for Biomaterials, Cellular, and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore, 632014, Tamil Nadu, India.
Drug Discovery Unit, Centre for Biomaterials, Cellular, and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore, 632014, Tamil Nadu, India.
Anal Biochem. 2022 Oct 15;655:114874. doi: 10.1016/j.ab.2022.114874. Epub 2022 Aug 24.
In the field of lateral flow assay (LFA), the application of aptamer as a bioreceptor has been implemented to overcome the limitations of antibodies, such as tedious in vivo processes, short shelf-life, and functionalization issues. To address these limitations aptamer-based LFA (ALFA) is preferred to antibody-based LFA that produces higher sensitivity and specificity. In principle, aptamers have a strong affinity towards their targets like small, large, and non-immunogenic molecules because of their high affinity, sensitivity, low dissociation constant, cost-effectiveness, and flexible nature. Thus, ALFA can be considered an efficient biosensor model for its superior portability, rapid detection with quick turnaround time, and usability by a non-technical person at any location with simple visual output. This review concisely overviews ALFA, its principles, formats, aptamer selection process, and biomedical applications. In addition, the critical components to design, develop, test, and amplify signals to create ALFA are discussed in brief. In addition, the aspects of conceptualization of ALFA product transforming from bench-side laboratory design and fabrication to commercial market are addressed in detail.
在侧向流动分析（LFA）领域，已采用适配体作为生物受体来克服抗体的局限性，如体内过程繁琐、保质期短和功能化问题。为解决这些局限性，基于适配体的LFA（ALFA）比基于抗体的LFA更受青睐，因为它能产生更高的灵敏度和特异性。原则上，适配体因其高亲和力、灵敏度、低解离常数、成本效益和灵活的性质，对其靶标（如小分子、大分子和非免疫原性分子）具有很强的亲和力。因此，ALFA因其卓越的便携性、快速检测且周转时间短以及非技术人员在任何地点均可通过简单的视觉输出进行使用，可被视为一种高效的生物传感器模型。本综述简要概述了ALFA、其原理、形式、适配体选择过程以及生物医学应用。此外，还简要讨论了设计、开发、测试和放大信号以创建ALFA的关键组件。此外，还详细阐述了从实验室台面设计和制造到商业市场的ALFA产品概念化方面。
