Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang 13200, Malaysia.
Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang 13200, Malaysia.
Talanta. 2019 Jul 1;199:556-566. doi: 10.1016/j.talanta.2019.02.066. Epub 2019 Feb 18.
Aptamers are nucleic acid-based molecular recognition elements that are specific and have high binding affinity against their respective targets. On account of their target recognition capacity, aptamers are widely utilized in a number of applications including diagnostics. This review aims to highlight the recent developments of aptasensors expedient for point-of-care (POC) diagnostics. Significant focus is given on the primary assay formats of aptamers such as fluorescence, electrochemical, surface plasmon resonance (SPR) and colorimetric assays. A potpourri of platforms such as paper-based device, lateral flow assay, portable electrodes, portable SPR and smart phones expedient for point-of-care (POC) diagnostics are discussed. Emphasis is also given on the technicalities and assay configurations associated with the sensors.
适体是一种基于核酸的分子识别元件，对其各自的靶标具有特异性和高亲和力。由于其靶标识别能力，适体广泛应用于许多领域，包括诊断。本综述旨在强调适体在即时诊断（POC）方面的最新发展。重点介绍了适体的主要分析方法，如荧光、电化学、表面等离子体共振（SPR）和比色分析。讨论了各种适用于即时诊断（POC）的平台，如基于纸的设备、侧向流动分析、便携式电极、便携式 SPR 和智能手机。还强调了与传感器相关的技术和分析配置。
