The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, 266237, China.
College of Horticulture, College of Life Sciences, Hai xia Institute of Science and Technology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.
Nat Commun. 2022 Aug 26;13(1):5040. doi: 10.1038/s41467-022-32770-7.
Stomatal pores allow gas exchange between plant and atmosphere. Stomatal development is regulated by multiple intrinsic developmental and environmental signals. Here, we show that spatially patterned hydrogen peroxide (HO) plays an essential role in stomatal development. HO is remarkably enriched in meristemoids, which is established by spatial expression patterns of HO-scavenging enzyme CAT2 and APX1. SPEECHLESS (SPCH), a master regulator of stomatal development, directly binds to the promoters of CAT2 and APX1 to repress their expression in meristemoid cells. Mutations in CAT2 or APX1 result in an increased stomatal index. Ectopic expression of CAT2 driven by SPCH promoter significantly inhibits the stomatal development. Furthermore, HO activates the energy sensor SnRK1 by inducing the nuclear localization of the catalytic α-subunit KIN10, which stabilizes SPCH to promote stomatal development. Overall, these results demonstrate that the spatial pattern of HO in epidermal leaves is critical for the optimal stomatal development in Arabidopsis.
气孔允许植物和大气之间进行气体交换。气孔的发育受多种内在发育和环境信号的调节。在这里，我们表明空间模式的过氧化氢（HO）在气孔发育中起着至关重要的作用。HO 在分生组织细胞中高度富集，这是由 HO 清除酶 CAT2 和 APX1 的空间表达模式建立的。SPCH（气孔发育的主要调节因子）直接结合 CAT2 和 APX1 的启动子，抑制它们在分生组织细胞中的表达。CAT2 或 APX1 的突变导致气孔指数增加。由 SPCH 启动子驱动的 CAT2 的异位表达显著抑制了气孔的发育。此外，HO 通过诱导催化 α 亚基 KIN10 的核定位来激活能量传感器 SnRK1，从而稳定 SPCH 以促进气孔发育。总的来说，这些结果表明，表皮叶片中 HO 的空间模式对于拟南芥最佳气孔发育至关重要。
