The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel.
The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel.
Adv Drug Deliv Rev. 2022 Nov;190:114460. doi: 10.1016/j.addr.2022.114460. Epub 2022 Aug 27.
Expanding the chemical repertoire of natural and artificial protein-based polymers (PBPs) can enable the production of sequence-defined, yet chemically diverse, biopolymers with customized or new properties that cannot be accessed in PBPs composed of only natural amino acids. Various approaches can enable the expansion of the chemical repertoire of PBPs, including chemical and enzymatic treatments or the incorporation of unnatural amino acids. These techniques are employed to install a wide variety of chemical groups-such as bio-orthogonally reactive, cross-linkable, post-translation modifications, and environmentally responsive groups-which, in turn, can facilitate the design of customized PBP-based drug-delivery systems with modified, fine-tuned, or entirely new properties and functions. Here, we detail the existing and emerging technologies for expanding the chemical repertoire of PBPs and review several chemical groups that either demonstrate or are anticipated to show potential in the design of PBP-based drug delivery systems. Finally, we provide our perspective on the remaining challenges and future directions in this field.
扩展天然和人工蛋白质基聚合物（PBPs）的化学组成，可以生产具有定制或新性能的序列定义但化学组成多样化的生物聚合物，而这些性能是仅由天然氨基酸组成的 PBPs 无法获得的。各种方法可以扩展 PBPs 的化学组成，包括化学和酶处理或非天然氨基酸的掺入。这些技术用于安装各种化学基团，如生物正交反应性、可交联、翻译后修饰和环境响应基团，这些基团反过来又可以促进设计具有改进、微调或全新性能和功能的定制基于 PBP 的药物递送系统。在这里，我们详细介绍了扩展 PBPs 化学组成的现有和新兴技术，并回顾了一些已经展示或预计在基于 PBP 的药物递送系统设计中具有潜力的化学基团。最后，我们对该领域的剩余挑战和未来方向提供了我们的看法。
