Department of Human Anatomy, Jilin Medical University, Jilin City 132013, Jilin Province, China.
Comput Intell Neurosci. 2022 Aug 5;2022:2345039. doi: 10.1155/2022/2345039. eCollection 2022.
To explore the changes of pain sensitivity (PS) in the masseter area (MA) in the rat model of psychological stress and the mechanism of action between spinal nucleus neurons and astrocytes in the trigeminal ganglion. The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated "cross" maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1 and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group ( < 0.01). In addition, the activity level and serum CORT and ACTH levels AND mean pain threshold in the MA of groups B and C were greatly lower than control group ( < 0.05). The activation rate of GFRP in group C ( < 0.01) and the protein expression of IL-1 and IL-1RI ( < 0.05) in rat trigeminal ganglion astrocytes of groups B and C was greatly higher than control group, indicating the increase of RS time, the release of IL-1 and IL-1RI can activate neurons and astrocytes in spinal trigeminal nucleus (STN) nerve and increase the PS of the MA.
探讨心理应激大鼠模型中咀嚼肌区（MA）痛觉敏感性（PS）的变化，以及三叉神经节（TG）中脊髓核神经元与星形胶质细胞之间的作用机制。
将 40 只 Sprague-Dawley 大鼠随机分为对照组（无处理）、A 组（束缚应激 1 d）、B 组（束缚应激 7 d）和 C 组（束缚应激 14 d）。比较各组大鼠体重增长率（WGR），采用 ELISA 法分析血清中 CORT 和 ACTH 的差异。采用旷场试验和高架十字迷宫试验检测大鼠的行为变化。最后，检测大鼠 MA 的痛阈、脑组织延髓部分星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）的激活量和白细胞介素 1（IL-1）和白细胞介素 1 受体 I（IL-1RI）的蛋白表达。
7 d 和 14 d 时的 WGR 明显低于对照组（ < 0.01）。此外，B 组和 C 组的活动水平和血清 CORT 和 ACTH 水平以及 MA 的平均痛阈明显低于对照组（ < 0.05）。C 组的 GFAP 激活率（ < 0.01）和 B 组和 C 组大鼠三叉神经节星形胶质细胞中 IL-1 和 IL-1RI 的蛋白表达（ < 0.05）明显高于对照组，表明 RS 时间的延长，IL-1 和 IL-1RI 的释放可激活脊髓三叉神经核（STN）神经中的神经元和星形胶质细胞，增加 MA 的 PS。
