Xia Wuzheng, Zeng Cong, Zheng Zehao, Huang Chunwang, Zhou Yu, Bai Lan
Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital/The First School of Clinical Medicine, Southem Medical University, Guangzhou, China.
Department of Organ Transplant, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Front Cell Dev Biol. 2022 Aug 12;10:844759. doi: 10.3389/fcell.2022.844759. eCollection 2022.
Mitochondrion and ferroptosis are related to tumorigenesis and tumor progression of hepatocellular carcinoma (HCC). Therefore, this study focused on exploring the participation of lncRNAs in mitochondrial dysfunction and ferroptosis using public datasets from The Cancer Genome Atlas (TCGA) database. We identified the mitochondrion- and ferroptosis-related lncRNAs by Pearson's analysis and lasso-Cox regression. Moreover, real-time quantitative reverse transcription PCR (RT-qPCR) was utilized to further confirm the abnormal expression of these lncRNAs. Based on eight lncRNAs, the MF-related lncRNA prognostic signature (LPS) with outstanding stratification ability and prognostic prediction capability was constructed. In addition, functional enrichment analysis and immune cell infiltration analysis were performed to explore the possible functions of lncRNAs and their impact on the tumor microenvironment. The pathways related to G2M checkpoint and MYC were activated, and the infiltration ratio of regulatory T cells and M0 and M2 macrophages was higher in the high-risk group. In conclusion, these lncRNAs may affect mitochondria functions, ferroptosis, and immune cell infiltration in HCC through specific pathways, which may provide valuable insight into the progression and therapies of HCC.
线粒体与铁死亡与肝细胞癌（HCC）的肿瘤发生和肿瘤进展相关。因此，本研究利用来自癌症基因组图谱（TCGA）数据库的公共数据集，重点探索长链非编码RNA（lncRNAs）在线粒体功能障碍和铁死亡中的作用。我们通过Pearson分析和套索- Cox回归鉴定了与线粒体和铁死亡相关的lncRNAs。此外，利用实时定量逆转录PCR（RT-qPCR）进一步证实了这些lncRNAs的异常表达。基于8种lncRNAs，构建了具有出色分层能力和预后预测能力的MF相关lncRNA预后特征（LPS）。此外，进行了功能富集分析和免疫细胞浸润分析，以探索lncRNAs的可能功能及其对肿瘤微环境的影响。与G2M检查点和MYC相关的通路被激活，高危组中调节性T细胞以及M0和M2巨噬细胞的浸润率更高。总之，这些lncRNAs可能通过特定途径影响HCC中的线粒体功能、铁死亡和免疫细胞浸润，这可能为HCC的进展和治疗提供有价值的见解。
